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RNAi knock down of Dnmt3 in honey bee larvae

ABSTRACT: Effects of Dnmt3 dsRNA silencing on the development of honeybee larvae.

INSTRUMENT(S): GenePix Professional 4200A [Axon Instruments]

ORGANISM(S): Apis mellifera

SUBMITTER: Robert Kucharski

PROVIDER: E-MEXP-1394 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nutritional control of reproductive status in honeybees via DNA methylation.

Kucharski R R Maleszka J J Foret S S Maleszka R R

Science (New York, N.Y.) 20080313 5871

Fertile queens and sterile workers are alternative forms of the adult female honeybee that develop from genetically identical larvae following differential feeding with royal jelly. We show that silencing the expression of DNA methyltransferase Dnmt3, a key driver of epigenetic global reprogramming, in newly hatched larvae led to a royal jelly-like effect on the larval developmental trajectory; the majority of Dnmt3 small interfering RNA-treated individuals emerged as queens with fully developed ...[more]

PMID: 18339900

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