Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of 10-day old Arabidopsis seedlings treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin)Auxin induced effects investigated using bead-purified probe arrays to assess the technology

ABSTRACT: 21120 Arabidopsis thaliana gene sequence tags were amplified and purified using a novel bead-based strategy (see Publication for more details).This experiment was carried out to demonstrate the use of these arrays for expression profiling of Arabidopsis thaliana.Briefly, 10-day old Arabidopsis seedlings were treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin) and the effects on gene expression were analysed.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Morten Lukacs

PROVIDER: E-MEXP-140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana.

Wirta Valtteri V Holmberg Anders A Lukacs Morten M Nilsson Peter P Hilson Pierre P Uhlén Mathias M Bhalerao Rishikesh P RP Lundeberg Joakim J

BMC biotechnology 20050203

<h4>Background</h4>Transcriptional profiling using microarrays has developed into a key molecular tool for the elucidation of gene function and gene regulation. Microarray platforms based on either oligonucleotides or purified amplification products have been utilised in parallel to produce large amounts of data. Irrespective of platform examined, the availability of genome sequence or a large number of representative expressed sequence tags (ESTs) is, however, a pre-requisite for the design and ...[more]

PMID: 15689241

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Project description:The purpose of this experiment was to do investigatge the transcriptional effect of anti-TNF treatment in patients suffering from rheumatoid arthritis. Two series of hybridizations were performed. Each hybridization was done with a technical replicate (the same amount of RNA taken from the same amplified RNA aliquot labeled in two separate reactions and hybridized onto two separate arrays). In the first series of hybridizations RNA exptracted from biopsies taken before treatment was hybridized in a common reference design to enable comparisons between patients. In the second series RNA from biopsies taken after and before treatment were hybridized on the same chip in a direct design to enable investigation of the effect of treatment. 10 patients (8 females and 2 males, median age 54, range 25-69) meeting the American College of Rheumatology (ACR) criteria for RA were recruited for this study. All patients received infliximab (an antibody directed against TNF-alpha) infusions 3mg/kg at week 0 and than after 2 weeks and 6 weeks. Five of these patients were receiving prednisolon and all ten patients were treated with methotrexate to a maximum of 20 mg per week. Synovial biopsies were obtained during arthroscopy from all patients before and after a median of 9 weeks of treatment (with one exception, patient 7 where the second biopsy was obtained during open surgery). Biopsies were taken at the site of inflammation close to cartilage (cc) or not close to cartilage (ncc), defined as less or more than 1.5 cm away from cartilage, respectively. One biopsy was taken before and after treatment from patient 5, 7, 9 and 10. Multiple biopsies were retrieved before and/or after treatment from patient 1, 2, 3, 4, 6 and 7. Due to small amounts of RNA available for patient 9 it was only used in the second series of hybridizations. The average biopsy weight was 22 mg. The ethical committee at the Karolinska Hospital approved all experiments on human cells and tissues. Informed consent was obtained from all study subjects.

Dataset Information

Transcription profiling of 10-day old Arabidopsis seedlings treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin)Auxin induced effects investigated using bead-purified probe arrays to assess the technology

Publications

Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana.

Similar Datasets

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