Transcription profiling of melanized domamine neurons isolated from male and female patients with Parkinson disease to investigate gender differences

ABSTRACT: We used 500 melanized dopamine neurons isolated from post-mortem human substantia nigra pars compacta of each sample with laser capture microdissection. Total RNA was extracted with Arcturus PicoPure RNA extraction kit and amplified two times with Arcturus RiboAmp RNA amplification kit. the samples were then sent to the Partners Center for Genetics and Genomics (HPCGG.org) for labeling, hybridization and scanning according to standard Affymetrix protocols. Samples from a total of 16 human brains, 8 PDs (4 male and 4 female) and 8 controls (4 male and 4 female) were studied. These were selected from available material after screening total RNA extracted from a small region of the SN for integrity of 18S and 28S species using an Agilent bioanalyzer. We also matched the cases for age and post

ORGANISM(S): Homo sapiens

SUBMITTER: Ippolita Cantuti-Castelvetri 

PROVIDER: E-MEXP-1416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress