Transcription profiling of roots from wild type and WRKY25 mutant Arabidopsis plants after salt stress to identify AtWRKY25 target genes

ABSTRACT: Arabidopsis thaliana, wild-type (Col-0) and a T-DNA insertion mutant of WRKY25 gene(SAIL_529_B11, designated as wrky25-1),were hydroponically cultured for 20 d and treated with 1/2 x MS supplemented with 150 mM NaCl for 6 h. Roots were harvested and RNA samples were prepared. QiagenOperon oligonucleotide microarray(v1.0.3) was used to identify differentially expressed genes in wrky25-1 mutant compared to wild-type under salt stress treatment. Microarray profilings were performed with three independent biological replicates prepared at separate time.

INSTRUMENT(S): GenePix 4000B [Axon Instruments], arrayWoRx Biochip Reader [Applied Precision]

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yuanqing Jiang 

PROVIDER: E-MEXP-1429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress