Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human NHEK cells response to 2mM N-Acetyl-L-cystein (NAC) treatment - 1,12, 24 hour time-series


ABSTRACT: NHEK cells were plated at a density of 8 x 10 000/cm2 and the cell cultures were grown for 24 hours before addition of 2 mM N-Acetyl-L-Cystein. RNA obtained from cultures grown for 1, 12 and 24 hrs after NAC treatment were compared to RNA from untreated cells at the corresponding time points. I.e 1 hour NAC treated vs 1 hour untreated cells etc. Each EXTRACT represents an individual mRNA extraction and subsequent cDNA synthesis from a batch of totalRNA originating from one cellculture dish.

ORGANISM(S): Homo sapiens

SUBMITTER: Anna Gustafsson 

PROVIDER: E-MEXP-146 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Global gene expression analysis in time series following N-acetyl L-cysteine induced epithelial differentiation of human normal and cancer cells in vitro.

Gustafsson Anna C AC   Kupershmidt Ilya I   Edlundh-Rose Esther E   Greco Giulia G   Serafino Annalucia A   Krasnowska Eva K EK   Lundeberg Thomas T   Bracci-Laudiero Luisa L   Romano Maria-Concetta MC   Parasassi Tiziana T   Lundeberg Joakim J  

BMC cancer 20050707


<h4>Background</h4>Cancer prevention trials using different types of antioxidant supplements have been carried out at several occasions and one of the investigated compounds has been the antioxidant N-acetyl-L-cysteine (NAC). Studies at the cellular level have previously demonstrated that a single supplementation of NAC induces a ten-fold more rapid differentiation in normal primary human keratinocytes as well as a reversion of a colon carcinoma cell line from neoplastic proliferation to apical-  ...[more]

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