Transcription profiling of procyclic Trypanosoma brucei cultured at 27oC or under heat shock stress conditions at 41oC

ABSTRACT: Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to one volume medium that had been heated to 53°C and incubated at 41ºC for 60 minutes in a waterbath in a closed tube (41ºC sample). The control cells were added to one volume medium at 27ºC and also incubated for 60 minutes in a closed tube at 27°C. Cells were harvested and washed once in PBS. The harvesting was done within 8 minutes.

INSTRUMENT(S): ScanArray 4000XL [PerkinElmer]

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Rafael Queiroz 

PROVIDER: E-MEXP-1476 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress