Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin immunopreciptiation to determine the genome wide locatio of different components of the RNA polymerase II preinitiation complex in yeast

ABSTRACT: Genome wide location of different components of RNA polymerase II preinitiation complex in yeast S.cerevisiae

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yad Ghavi-helm

PROVIDER: E-MEXP-1502 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Mediator-dependent recruitment of TFIIH modules in preinitiation complex.

Esnault Cyril C Ghavi-Helm Yad Y Brun Sylvain S Soutourina Julie J Van Berkum Nynke N Boschiero Claire C Holstege Frank F Werner Michel M

Molecular cell 20080801 3

In vitro, without Mediator, the association of general transcription factors (GTF) and RNA polymerase II (Pol II) in preinitiation complexes (PIC) occurs in an orderly fashion. In this work, we explore the in vivo function of Mediator in GTF recruitment to PIC. A direct interaction between Med11 Mediator head subunit and Rad3 TFIIH subunit was identified. We explored the significance of this interaction and those of Med11 with head module subunits Med17 and Med22 and found that impairing these i ...[more]

PMID: 18691966

Similar Datasets

Transcription profiling by array of Lotus japonicus leaves from wild type and Ljgln2-2 mutant plants exposed to drought.

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described previously (Orea et al., 2002; Mï¿½rquez et al., 2005; Betti et al., 2006). The mutant progeny of two consecutive back-crosses with WT was used for the present work. WT and mutant seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18ï¿½C, with a photosynthetic photon flux density of 250 ï¿½mol/m2ï¿½s and a constant humidity of 70%. CO2 was automatically injected to a final concentration of 0.7% (v/v), to allow for normal growth of Ljgln2-2 mutant in a photorespiration suppressed atmosphere. Plants were watered with Hornum nutrient solution (Handberg & Stougaard, 1992). Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2011-04-29 | E-MEXP-2690 | biostudies-arrayexpress

Transcription profiling of rat heart transplants from Lewis to Lewis and Lewis to F344 strains with and without cold storage / prolonged cold ischemia.

Project description: Annual heart allograft failure in humans rates about 3-5%. The main reason after the first postoperative year is chronic rejection. Myointimal hyperplasia, the hellmark of chronic rejection, results in a specific type of ischemic heart disease. The lack of angina pectoris symptoms allow ventricular arrythmias, sudden cardiac death or heart failure to occur without warning. In addition, diagnostic tools such as endomyocardial biopsy, coronary angiography or intracoronary ultrasound fail to predict the individual risk for myocardial dysfunction. The mechanisms responsible for chronic rejection are predominantly alloimmune mediated with activated T cells, macrophages, B cell mediated antibody formation and secreted cytokines responding to HLA and other endothelial cell antigens. In addition, non immunologic risk factors such as recipient age, metabolic factors, hypertension and ischemia contribute to development of this disease. Previous studies have demonstrated that ischemia has a profound influence on short term allograft survival but the underlaying mechanisms remain largely unknown. Apoptosis seems to play a crucial role in ischemia/reperfusion injury and several mechanisms for programmed cell death have been described. However, consequences on long term cell function of viability have not been investigated. The aim of this study was to investigate the implication and the mechanism of prolonged cold organ storage as a non immunologic risk factor in the pathogenesis of chronic rejection in a cardiac allograft model. We aimed for answering the following specific questions: How does cold ischemia affect the alloimmue response short and long term? How does prolonged cold ischemia affect gene expression at later time points after transplantation? Does it influence gene expression during chronic rejection?

2010-10-13 | E-MEXP-2301 | biostudies-arrayexpress

Transcription profiling of human dermal fibroblasts and lung microvascular endothelial cells irradiated with protons

Project description:Normal human dermal fibroblasts (NHDF) and human lung microvascular endothelial cells (HMVEC-L) were irradiated with protons (0, 0.5, 1 and 2 Gy, 1GEv/n) at Brookhaven National Labs (BNL). Aim of the study is to find differentially transcribed genes in dependence of radiation dose/source and cell type.

2009-04-25 | E-TABM-379 | biostudies-arrayexpress

Transcription profiling of human CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

Project description:analysis of differential gene expression in CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

2009-08-14 | E-TABM-754 | biostudies-arrayexpress

Functional link between TFIIB and Mediator

Project description:In this study, we generate genomic maps of Mediator, Pol II, TBP, TFIIH, TFIIA, TFIIB, TFIIE, TFIIF, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae and in a mutant for the Mediator essential subunit Med10

2017-01-24 | E-MTAB-4607 | biostudies-arrayexpress

Transcription profiling of human embryonic kidney cell line HEK293T to investigate the effects of overexpression of FoxO4 or mutated FoxO4 in presence or absence of p300

Project description:Effects of overexpression of FoxO4 or mutated FoxO4 in presence or absence of p300 overexpression

2009-07-30 | E-MTAB-4 | biostudies-arrayexpress

Functional link between Rad2 and Mediator

Project description:In this study, we generate genomic maps of Mediator, Rad2, Pol II, TBP and TFIIH, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae. A related study involving ChIP-chip analysis of Rad2 occupany is also deposited at ArrayExpress under accession number E-MEXP-3875 ( http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3875 ).

2013-12-01 | E-MTAB-1595 | biostudies-arrayexpress

Mediator orchestrates PIC assembly

Project description:In this study, we generate genomic maps of Mediator, Pol II, TBP and TFIIH, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type (WT) strains and med17-ts mutants from Saccharomyces cerevisiae. Some of the data, concerning WT strains are also deposited at ArrayExpress under accession number E-MTAB-1595 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1595). There are 2 series of experiment: 1- WT (see E-MTAB-1595) and mutants med17-98, med17-444, and med17-670 (this submission) 2- WT and mutant med17-444 (this submission).

2015-09-03 | E-MTAB-2961 | biostudies-arrayexpress

Rad2 ChIP-chip experiment

Project description:This aim of this experiment is to assess the genome-wide Rad2 occupancy in yeast Saccharomyces cerevisiae by ChIP-chip, in the absence of exogenous genotoxic stress. A related study involving ChIP-seq analysis of Rad2 occupany is also deposited at ArrayExpress under accession number E-MTAB-1595 ( www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1595 ).

2013-12-01 | E-MEXP-3875 | biostudies-arrayexpress

RNA polymerase II - Mediator interaction

Project description:Comparison of genome-wide RNA polymerase II occupancy in wild-type and rpb3-2 yeast strains

2011-10-26 | E-MEXP-3043 | biostudies-arrayexpress