Chromatin immunopreciptitation of Drosophila DNA using antibodies against RMA polymerase II CTD repeats

ABSTRACT: Crosslinked chromatin from wild type flies was sonicated into small fragments, and used in ChIP reactions using a monoclonal antibody, 8WG16, against RNA polymerase II CTD repeats. Input material was removed prior to chIP, and a control chIP was incubated with normal rabbit IgG. The Input and IPed DNA was purified, amplified, and hybridized to Affymetrix Drosophila genomic tiling arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Cizhong Jiang 

PROVIDER: E-MEXP-1520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress