Transcription profiling of T. brucei cell lines over-expressing wild type RAB5A or mutant versions of RAB5A or RAB5B locked in the GTP-bound state

ABSTRACT: As a test of trypanosome transcriptome responsiveness, we analysed the effects of over-expression of the early endosome small GTPase Rab5A in procyclic form T.brucei, which results in significant augmentation of fluid phase and receptor-mediated endocytic activity (Pal et al. 2002).

part 1: 2 PCFWT and 2 PCF5AWT-OE (PCF cell lines over-expressing a wildtype version of RAB5A) biological replicates, as well as dye swaps were used.

part 2: 2 PCFWT and 2 PCF5AQL-OE (PCF cell lines over-expressing a mutant version of RAB5A locked in the GTP-bound state) biological replicates, as well as dye swaps were used.

part 3: 2 PCFWT and 2 PCF5BQL-OE (PCF cell lines over-expressing a mutant version of RAB5B locked in the GTP-bound state) biological replicates, as well as dye swaps wer

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Lila Koumandou 

PROVIDER: E-MEXP-1539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress