Transcription profiling of human differentiating cord blood derived megakaryocytes
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ABSTRACT: Ex vivo differentiation of megakaryocytes (Mk) was carried out using human cord blood (CB) CD34+ cells under the stimulation of recombinant human interleukin-3, stem cell factor, and thrombopoietin for 7 days, followed by thrombopoietin treatment alone for further 3 days. Total cellular RNA was extracted from Day-0 CD34+, Day-10 CD41+ and CD41- cells, respectively. Microarray was performed and the data was analyzed using the GeneChip Operating Software, Spotfire DecisionSite Software and Genomatix Application Software.
ORGANISM(S): Homo sapiens
SUBMITTER: CHE KANG LIM
PROVIDER: E-MEXP-1546 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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