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Transcription profiling of mouse heart tissue from animals exposed to mainstream tobacco smoke

ABSTRACT: Male C57B1/CBA mice were exposed to mainstream tobacco smoke (MTS) from two cigarettes daily, 5 days/week for 6 or 12 weeks. Mice were sacrificed immediately, or six weeks following, the last cigarette. High density DNA microarrays were used to characterize global gene expression changes in whole heart.

Western blot and ELISA assays were used to determine levels of Cyp1A1 protein in heart microsomes and total and active PAI-1 protein from tissue extracts.

ORGANISM(S): Mus musculus

SUBMITTER: Lynn Berndt

PROVIDER: E-MEXP-1553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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