Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SiRNAi knockdown of ZBTB4 in human SH-EP cells reveals Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation

ABSTRACT: SH-EP cells were depleted for ZBTB4; Transfection of the cells were performed by oligofectamin RNAimax (Invitrogen) with siRNA smart pool (Dharmacon RNA Technologies); total RNA was isolated using RNAeasy Mini Kit (QIAGEN), preamplified, labeled with Cy3 and Cy5, respectively, and hybridized to cDNA-microarrays

ORGANISM(S): Homo sapiens

SUBMITTER: Birgit Samans

PROVIDER: E-MEXP-1554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouseB-cell lymphomas with defined genetic alterations in p53 or p73

Project description:Induction of apoptosis by the tumor suppressor p53 is known to protect from Myc-driven lymphomagenesis. The p53 family member p73 is also a pro-apoptotic protein, which is activated in response to oncogenes like Myc. We therefore investigated whether p73 provides a similar protection from Myc-driven lymphomas as p53. To generate B-cell lymphomas with defined genetic alterations in p53 or p73, we crossed the Eµ-Myc transgenic to mice heterozygous for germ-line deletions in p53 (p53+/) or p73 (p73+/-). Lymphomas which have spontaneously developed in Eµ-Myc transgenic animals with the genotypes p53+/+, p53+/-, p73+/+, p73+/- or p73-/- were isolated when the animals were moribund and further processed for gene expression profiling with 22.5K cDNA microarrays.

2008-12-05 | E-MEXP-1620 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and myo mutant shoot apices

Project description:Transcription profiling of shoot apices of the myo mutant was compared with shoot apices of WT (background Ler).

2009-07-22 | E-MEXP-1735 | biostudies-arrayexpress

Transcription profiling of human Soas-2 cells infected with adenoviruses expressing p53 proteins with charge-neutralizing or charge-inverting mutations in the H1 helix to investigate the role of the dimerization interface in p53 function

Project description:To investigate the role of the dimerization interface for p53 function, we introduced modest charge-neutralizing (R181→L "EL") and more severe charge-inverting (R181→E "EE" and E180→R, R181→E "RE") mutations into the H1 helix of the full-length p53 molecule. To characterize the nuclear transcriptional function of p53 mutants in an unbiased manner we employed gene expression profiling using cDNA microarrays. Saos-2 cells were infected with adenoviruses expressing the p53 proteins EE, EL, WT and RE which span the entire spectrum of apoptotic activity. Total cellular RNA was isolated 18 hours after infection when apoptosis had not yet occurred.

2010-04-01 | E-MEXP-1209 | biostudies-arrayexpress

Transcription profiling time series of mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, starved for 72 h and afterwards stimulated with EGF

Project description:Signaling pathways specifically induced by the oncogenic receptor Xmrk can be elucidated by comparing gene expression in cells with the activated receptor to gene expression in cells with the non-activated receptor. Mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, were starved for 72 h and afterwards stimulated with EGF. We compared cells stimulated for 15 minutes, 1 h, 2 h, 4 h, 8 h or 24 h, respectively, to unstimulated cells.

2008-08-31 | E-MEXP-1311 | biostudies-arrayexpress

Transcription profiling of mouse embryonic fibroblasts carm1-/- completemented with CARM1 wt were left untreated or were stimulated for 3hours with 10ng/ml mTNFa

Project description:Complemented carm1-/- MEFs with CARM1 wt were left untreated or were stimulated for 3hours with 10ng/ml mTNFa. mTNFa-induced NF-kB dependent gene expression was analyzed on a custom array for specific NF-kB target genes

2009-09-01 | E-MEXP-2020 | biostudies-arrayexpress

Transcription profiling of human Wilms tumor samples to select genes responsible for tumor development, metastasis and progression

Project description:Expression analysis was performed in 77 Wilms tumors in order to select genes responsible for tumor development, metastasis and progression.

2009-02-05 | E-MEXP-221 | biostudies-arrayexpress

Transcription profiling of skeletal muscle from leptin treated wild type and obese mice

Project description:Wild type (C57BL/6J) mice were divided in control (W), leptin-treated (E) and pair-fed (F). Obese (ob/ob) mice were divided in control (O), leptin-treated (L) and pair-fed (P). Control (W and O) and pair-fed animals (F and P) received vehicle (PBS), while E and L groups were intraperitoneally administrated with leptin for 28 days. Control (W and O) and leptin-treated (E and L) groups were provided with water and food ad libitum with, while daily food intake of pair fed (F and P) groups were matched to the amount eaten by the leptin-treated groups (E and L) the day before to discriminate the inhibitory effect of leptin on appetite.

2009-09-26 | E-MEXP-1831 | biostudies-arrayexpress

Comparative genomic hybridization of human glioblastoma biopsies and reference DNA to identify chromosomal abberrations

Project description:DNA from 5 different Glioblastoma biopses were tested for chromsomal aberrations against reference DNA

2008-12-31 | E-MEXP-1562 | biostudies-arrayexpress

Transcription profiling of Citrus clementina flavedo mutant 39B3 which shows a delay in fruit color change

Project description:Transcriptomic profiling of the flavedo of the Citrus clementina mutant 39B3, showing a delay in fruit colour change.

2011-03-23 | E-MEXP-2638 | biostudies-arrayexpress

Transcription profiling of Citrus clementina flavedo mutant 39E7 which shows a delay in fruit color change

Project description:Transcriptomic approach to the study of the Citrus clementina mutant 39E7, showing a delay in fruit colour change.

2011-03-23 | E-MEXP-2641 | biostudies-arrayexpress