Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

5 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of rat hair and supporting cells from the cristae ampullaris in the inner ear

ABSTRACT: Using laser capture microdisection, the more superficial layer of the cristae ampullaris sensory epithelium encompassing types I and II hair cells, and the supporting cell cytoplasm interspersed between them was captured in the first pass, and the underlying supporting cell bodies resting on the basal lamina were captured in a second pass. Individual hair cells were identified under light microscopy at 200X magnification by their stereociliary tuft and the calyceal nerve ending surrounding them and the presence of their nucleus in the outermost portion of the epithelia.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Christy Erbe

PROVIDER: E-MEXP-1559 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of rat calyceal vestibular primary afferent neurons (calretinin positive) and dimorphic-bouton vestibular primary afferent neurons (calretinin negative)

Project description:Pooled Immunoflourescent guided laser capture microdisection of calyceal vestibular primary afferent neurons (calretinin positive) and dimorphic-bouton vestibular primary afferent neurons (calretinin negative) were used for microarray expression profiling. Transcription analysis of each of these biologically diverse pools was completed.

2008-05-01 | E-MEXP-1560 | biostudies-arrayexpress

Transcription profiling of human CD133 and CD34 positive hematopoietic stem cells imuno-magnetically isolated from bone marrow and umbilical cord blood

Project description:Expression Profiling of CD133 and CD34 positive Hematopoietic Stem Cells imuno-magnetically isolated from Bone Marrow and Umbilical Cord Blood

2009-07-31 | E-MEXP-1890 | biostudies-arrayexpress

p53 binding in H24 cell line

Project description:Effects of p53 acetylation at the C terminal domain on p53 binding

2011-02-01 | E-MEXP-3027 | biostudies-arrayexpress

Transcription profiling of human monocyte-HIV interactions sampled from HIV patients at different disease stages vs controls

Project description:Ex vivo monocytes of HIV patients in different stages of the disease were compared with monocytes from healthy control donors.

2010-06-30 | E-MEXP-2255 | biostudies-arrayexpress

Chromatin immunoprecipitation to determine p53 binding to promoter regions in human U2OS cells without activation of p53

Project description:p53 binding to promoter regions in U2OS cells without activation of p53

2010-01-31 | E-MEXP-2537 | biostudies-arrayexpress

Chromatin immunoprecipitation of human SKBR3 cells using anti-p53 antibodies

Project description:ChIP with anti-p53 (DO-1) of human SKBR3 cells

2010-02-18 | E-MEXP-2538 | biostudies-arrayexpress

Transcription profiling of honey bee brain and ovary reveals pheromone response is correlated with reproductive traits and brain gene expression in individual honey bees

Project description:In honey bees (Apis mellifera), the reproductive queen produces a pheromonal signal that regulates many aspects of worker behavior and physiology and is critical for maintaining colony organization. Queen mandibular pheromone (QMP) inhibits worker reproduction, attracts workers from a short distance (retinue response), inhibits the rearing of new queens, modulates age-related division of labor and globally alters brain gene expression in worker bees. Interestingly, substantial variation in worker retinue responses to QMP has been found between colonies, but the molecular and physiological bases for variation in individual responses to the queen have not been characterized. Here, we demonstrate that individual retinue response is negatively correlated with traits associated with reproductive potential. Workers with low response to QMP have more ovarioles and higher levels of vitellogenin transcripts than workers with a high response to QMP, suggesting that workers with greater reproductive potential may be attempting to escape queen control. Retinue response appears to be associated with a suite of behavioral and physiological traits that may be pleiotropically linked. However, while these phenotypes are all correlated at the organismal level, the underlying brain expression patterns and gene networks associated with each trait are independent, suggesting that these phenotypes are uncoupled at the molecular level in adult bees. These studies provide insights into the ultimate and proximate causes of natural variation in pheromone response in honey bees.

2008-12-31 | E-MEXP-1724 | biostudies-arrayexpress

Transcription profiling time series of human endothelial cells invading into a three-dimensional collagen matrix

Project description:Gene expression profiling studies were performed at various times using a defined in vitro endothelial cell invasion assay system. Total RNA from invading cells was isolated at 0, 6, 12 and 18 hours and subjected to microarray analyses.

2008-10-01 | E-MEXP-1800 | biostudies-arrayexpress

Expression profiling of lesional and non-lesional bulge cells from Lichen planopilaris patients

Project description:Lichen planopilaris (LPP) is a chronic inflammatory disease of unknown pathogenesis that leads to permanent hair loss. Whilst destruction of epithelial hair follicle stem cells (eHFSCs) that reside in an immunologically protected niche of the HF epithelium, the bulge, is a likely key event in LPP pathogenesis, this remains to be demonstrated. Laser capture microdissection of bulge cells from biopsies of lesional and non-lesional scalp skin from adult LPP patients were analyzed by microarray analysis.

2013-07-04 | E-MEXP-3859 | biostudies-arrayexpress

Transcription profiling of rat sarcopenic skeletal muscle after treatment with extract of Ginko biloba Egb 761 shows that a juvenile gene expression profile is reactivated

Project description:The sarcopenia-associated decline of the muscular performances in older people is improved by exercise and caloric restriction but is still lacking a more suitable pharmacological treatment. We investigated the effects of EGb 761, a Gingko biloba extract used to treat chronic age-dependent neurological disorders, on the soleus muscle of aged rats. DNA microarray analysis shows that the EGb 761 treatment is associated with the transcriptional reprogramming of genes related to regeneration and energy production functions. EGb 761 up-regulated genes implicated in myogenesis though the TGF? signaling pathway. Also, EGb 761, by modulating genes implicated in fatty acids and glucose oxidation, reversed the agerelated shift from lipids to glucose and improved energy production in mitochondria. These modifications restore a more juvenile gene expression pattern that is concomitant with the improvement of the muscular performances, as assessed by electrophysiological and biochemical parameters. Thus, EGb 761 may represent a novel treatment for sarcopenia

2009-06-30 | E-MEXP-1937 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data