Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

25 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA profiling of liver from vitellogenic and non-vitellogenic zebrafish females

ABSTRACT: Liver samples were collected from zebrafish females (3 months old). Vitellogenic females were maintained under conditions of 14 h light: 10 h dark. Non-vitellogenic females were obtained by exposing females to 6 h light/18 h dark regime.

ORGANISM(S): Danio rerio

SUBMITTER: Amit Cohen

PROVIDER: E-MEXP-1572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Estrogen regulation of microRNAs, target genes, and microRNA expression associated with vitellogenesis in the zebrafish.

Cohen Amit A Smith Yoav Y

Zebrafish 20130615 5

Estrogen is a steroid hormone that has been implicated in a variety of cellular and physiological processes and in the development of diseases such as cancer. Here we show a remarkable widespread microRNA (miRNA) downregulation in the zebrafish (Danio rerio) liver following 17β-estradiol (E2) treatment. This unique miRNA expression signature in the fish liver was further supported by a combination of computational predictions with gene expression microarray data, showing a significant bias towar ...[more]

PMID: 23767875

Similar Datasets

Transcription profiling of human dendritic cells treated with lipopolysaccharides and R848

Project description:Toll-like receptor (TLR) signalling activation by pathogens is critical to the induction of immune responses, and demands tight regulation. Chemokine ligand 2 (CCL2) secretion triggered by TLR4 or TLR8 engagement is strongly inhibited upon simultaneous activation of both TLRs in human monocyte-derived dendritic cells (MD-DC). Impaired CCL2 secretion occurs concomitantly to IL-12 up-regulation, being part of a complex regulatory circuit ensuring optimal Th type 1 polarization. Interestingly, triggering selected TLRs or their combinations differently affects nuclear factor-kB p65 activation and microRNA expression. To investigate in details such different modulation we performed a microarray profiling of MD-DCs stimulated by different TLRs agonist or their combination in three different donors. We found that CCL2 supplies an important immunomodulatory role to DCs, and may contribute to dictate the cytokine profile in Th type 1 responses induced by DCs.

2009-05-22 | E-MEXP-2183 | biostudies-arrayexpress

MicroRNA profiling of adult male zebrafish exposed to 17-beta estradiol (E2) for 0, 1, 4. 12 and 24 hours

Project description:Microarrays displaying zebrafish miRNAs specific probes were hybridized using RNA samples originating from whole body homogenates of male zebrafish exposed to 17-beta estradiol (E2) for 0, 1, 4. 12 and 24 hours. 0 hr fish No. 40, 41, 42. 1hr fish No. 8, 10, 11. 4hr fish No. 19, 20, 23. 12hr fish No. 24, 25, 26. 24hr fish No. 29, 31, 32. <br><br> The hybridizations used in these experiments: Chip 1: 10-42, Chip 2: 40-8, Chip 3: 41-11, Chip 4: 42-19, Chip 5: 20-40, Chip 6: 23-41, Chip 7: 25-42, Chip 8: 40-24, Chip 9: 41-26, Chip 10: 42-29, Chip 11: 31-40, Chip 12: 32-41

2007-12-30 | E-MEXP-1147 | biostudies-arrayexpress

MicroRNA profiling of Drosophila head and thorax from females mated with sex peptide null or sex peptide producing males

Project description:Comparison of females mated to males null for Sex-Peptide (SP0, Liu, H. and E. Kubli, Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster. Proc Natl Acad Sci U S A, 2003. 100(17): p. 9929-33) or to control, Sex-Peptide producing, males. Comparisons were made at 3 and 6 hours after mating, in dissected Head - Thorax body parts.

2009-08-01 | E-MEXP-2079 | biostudies-arrayexpress

MicroRNA profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2080 | biostudies-arrayexpress

Transcription profiling of male zebra fish exposed to E2 and liver from non-vitellogenic and vitellogenic females to identify genes associated with vitellogenisis

Project description:Two experiments were preformed, one for evaluating the effect of E2 exposure in males and a second experiment for comparing gene expression in the liver of non-vitellogenic females with that of vitellogenic females. The experiments designs were as follows:<br>1) Four months old zebrafish were divided into three groups consisting of 8 fish in each group: i) males (N=8) were exposed to E2 (Sigma-Aldrich, Israel) by immersion for 48 h (group E). The concentration used was 5 ?g/L (18 nM), as 3-4 ng/ml was determined to be the E2 natural concentration in the plasma of adult vitellogenic female ZF (Heiden et al 2006); ii) untreated males(N=8); iii) untreated vitellogenic females(N=8). Four replicate samples were prepared for each group and each replicate consisted of a pooled sample from livers of two fish. <br>2) In order to reveal the differences between vitellogenic and non-vitellogenic females, a second experiment was designed. One month old zebrafish were divided into two groups consisting of 32 fish in each group. Due to the small size of the liver, pooled samples from eight fish were prepared for each of the four replicates in the expression studies. The groups consisted of: i) fish that were kept under a light/dark cycle of 14/10 h for 5 weeks (N=32). with ovaries in vitellogenic stage; ii) fish that were kept under a light/dark cycle of 6/18 h for the same time period (N=32) with non-vitellogenic ovaries.

2009-05-26 | E-MEXP-1683 | biostudies-arrayexpress

SNAI1 induced epithelial to mesenchymal transition miRNA study in time course

Project description:To identify miRNAs participating in SNAI1-orchestrated regulatory pathways, we analysed time-resolved microarray data of SNAI1-induced EMT, obtained during conditional expression of SNAI1 in a “Tet-Off” MCF7-SNAI1 breast carcinoma cell model (Vetter et al, 2009). miRNA time series study for 7 times points (4h, 8h, 12h, 24h, 48h, 72h, 96h) in 3 replicates. For each time point, we compared induced and non-induced samples. Overall, we have 42 samples (21 hybridizations).

2012-01-13 | E-GEOD-35074 | biostudies-arrayexpress

microRNA expression profiling of CML and normal quiescent cells.

Project description:Chronic myeloid leukaemia (CML) is a clonal haemopoietic stem cell (HSC) disorder associated with the BCR-ABL oncogene, which encodes a constitutively active tyrosine kinase. We have demonstrated the existence of CML HSC which are resistant to the tyrosine kinase inhibitors (TKI). We have hypothesised that CML stem cells are dependent on key survival pathways that are induced by TKI treatment. In order to elucidate these key survival pathways, we have investigated the transcriptional differences between normal and CML stem/progenitor cells (CD34+38-) and by carrying out RNA profiling for the different populations. CD34+38- cells were isolated from chronic phase patient samples. LCSciences human miRNA microarray chips were used (100% coverage of mature miRNAs listed in miRBase version 14).

2018-01-12 | E-MTAB-3220 | biostudies-arrayexpress

UV-B regulation of microRNAs in maize

Project description:Differential expression of microRNAs was studied in maize leaves after an 8-h-exposure under UV-B light. As a control, plants were kept in the greenhouse in the absence of UV-B 4-week maize plants were grown in the greenhouse in the absence of UV-B. Then, they were divided in two groups. One group was treated with UV-B lights provided once for 8 h, starting 3 h after the beginning of the light period, using fixtures mounted 30 cm above the plants (Phillips, F40UVB 40 W and TL 20 W/12) at a UV-B intensity of 2 W m-2, UV-A: 0.65 W m-2. The bulbs were covered with cellulose acetate to exclude wavelengths <280 nm. For the second control group, plants were exposed for 8 h under the same lamps covered with polyester film (no UV-B treatment, UV-B: 0.04 W m-2, UV-A: 0.4 W m-2). Lamp output was recorded using a UV-B/UV-A radiometer (UV203 A+B radiometer, Macam Photometrics, Ltd, Livingston, UK) to insure that both the bulbs and filters provided the designated UV dosage in all treatments. Leaf samples were collected immediately after irradiation. RNA was extracted immediately after the treatments, and used for the microRNA microarray experiments.

2013-10-31 | E-GEOD-19768 | biostudies-arrayexpress

miRNA profiling of mouse primary peritoneal macrophages infected with vesicular stomatitis virus (VSV)

Project description:To investigate whether and what miRNAs expression might be regulated by VSV (vesicular stomatitis virus?) challenge, we analyzed the miRNA expression profile of mouse primary peritoneal macrophages infected with VSV by using an array-based miRNA profiling. After the infection of VSV at MOI 10 for 48 h, the array revealed that many miRNAs were up-regulated in macrophages?

2009-05-11 | E-MEXP-2110 | biostudies-arrayexpress

Oogenesis in Solea senegalensis: Previtellogenic versus vitellogenic ovaries

Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from previtellogenic stage to vitellogenic stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (CerdÃ et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Pv-1, IR-Pv-2, IR-Pv-3 for previtellogenic individuals and IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals). For the experiment, the vitellogenic stage was used as the control.

2010-06-21 | E-GEOD-17336 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data