Transcription profiling of human freshly isolated adipose-derived adult stem cells (ADASCs) vs cultured cells

ABSTRACT: In order to assess whether culturing adipose-derived adult stem cells (ADASCs) affect their gene expression (see Sample Growth Condition Protocol), we wanted to identify possible genes that were differentially expressed between cultured polyclonal CD31- ADASCs and freshly isolated (uncultured) polyclonal CD31- ADASCs. To that end, RNA was isolated from cultured and uncultured ADASCs from three different donors and analyzed using the Affymetrix Microarray HG-U133A. Then, using the Affymetrix program MAS 5.0 we performed three comparisons and could identify differentially expressed transcripts common between the three donors, using the Affymetrix program DMT 3.0.

ORGANISM(S): Homo sapiens

SUBMITTER: Katrine Fronsdal 

PROVIDER: E-MEXP-168 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress