Transcription profiling of Streptococcus gordonii wildtype and Brf- deletion and insertion mutants
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ABSTRACT: RNA was isolated from early stationary phase S. gordonii V288(OD620 nm ~ 1.8).Bacteria were grown in chemically defined synthetic media (FMC) ( K2HPO4, 300 µg ml-1; KH2PO4 440 µg ml-1; Na2HPO4, 3.15 mg ml-1; NaH2PO4, 2.05 mg ml-1; (NH4)2SO4 0.6 mg ml-1; sodium citrate, 225 µg ml-1; riboflavin, 0.4 µg ml-1; biotin, 0.01 µg ml-1; folic acid, 0.1 µg ml-1; pantothenate, 0.8 µg ml-1; p-aminobenzoic acid 0.1 µg ml-1; thiamine 0.4 µg ml-1; nicotinamide 2.0 µg ml-1; pyridoxamine 0.8 µg ml-1; D-glucose 20 mg ml-1; sodium acetate 6 mg ml-1; adenine 35 µg ml-1; guanine µg ml-1; uracil µg ml-1; MgSO4, 200 µg ml-1; NaCl, 10 µg ml-1; FeSO4, 10 µg ml-1; MnSO4 10 µg ml-1; glutamine 5 µg ml-1; L-glutamic acid, 300 µg ml-1; L-valine, 100 µg ml-1; 110 µg ml-1 of L-lysine, L-asparate, L-isoleucine, L
ORGANISM(S): Streptococcus gordonii
SUBMITTER: Kelli Palmer
PROVIDER: E-MEXP-1712 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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