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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of liver-specific Hnf4alpha knockout and wild type mouse liver

ABSTRACT: Gene expression differences between wild-type and liver-specific Hnf4alpha knockout (Albumin Cre+/-; Hnf4 fl/fl) mouse liver were assessed using RNA obtained from 2-month-old control and genetically modified female mice. RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays [Mouse430_2].

ORGANISM(S): Mus musculus

SUBMITTER: Joan-Marc Servitja

PROVIDER: E-MEXP-1733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional targets of the monogenic diabetes transcription factors HNF-1alpha and HNF-4alpha are highly conserved between mice and humans.

Boj Sylvia F SF Servitja Joan Marc JM Martin David D Rios Martin M Talianidis Iannis I Guigo Roderic R Ferrer Jorge J

Diabetes 20090202 5

<h4>Objective</h4>The evolutionary conservation of transcriptional mechanisms has been widely exploited to understand human biology and disease. Recent findings, however, unexpectedly showed that the transcriptional regulators hepatocyte nuclear factor (HNF)-1alpha and -4alpha rarely bind to the same genes in mice and humans, leading to the proposal that tissue-specific transcriptional regulation has undergone extensive divergence in the two species. Such observations have major implications for ...[more]

PMID: 19188435

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Transcription profiling of mouse adult male wild-type liver (M-WT), adult female wild-type liver (F-WT), adult male liver-specific HNF4alpha knockout liver (M-KO) and adult female liver-specific HNF4alpha knockout liver (F-KO) to investigate sex-dependent and HNF4alpha-dependent gene expression

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