RNAi knock down of ADP-glucose pyrophosphorylase (AGP) in Pisum sativum developing cotyledons

ABSTRACT: During seed growth, sugar and nitrogen compounds confer regulatory control on storage activities. Thus, seed storage production could be regulated by the supply of nutrients. In order to improve nitrogen flux into the embryo, transgenic pea lines were created where ADP-glucose pyrophosphorylase (AGP) from Pisum sativum has been repressed by RNAi approach in the seeds under control of the seed-specific LeB4 promotor (Bäumlein et al. Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within legumin box is essential for tissue-specific expression of a legumin gene. Plant J 1992 2: 233-239). The plastidial enzyme AGP catalyzes the reaction of glucose-1-phosphate and ATP to pyrophosphate and ADP-glucose, which is the substrate for starch synthase. The AGP activity

ORGANISM(S): Pisum sativum

SUBMITTER: Kathleen Weigelt 

PROVIDER: E-MEXP-1749 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress