Project description:Comparative genome hybridization was used to study genomic diversity among S. suis strains in more detail. All strains were hybridized to an oligo-array based on the genome of P1/7 with P1/7 as an internal reference in all experiments.
Project description:Comparative genome hybridization was used to study genomic diversity among S. suis strains in more detail. All strains were hybridized to an oligo-array based on the genome of P1/7 with P1/7 as an internal reference in all experiments.
Project description:Transcriptome comparison of Streptococcus suis wildtype and argR knock-out strain grown to exponential or early-stationary phase, respectively.
Project description:Employing a transcriptomic approach, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite.
Project description:25 ml of a modified basal salts medium (BSM; Hareland et al. 1975, J Bacteriol 121:272) supplemented with 0.05% casamino acids (Becton & Dickinson, Sparks, USA), 0.05 % yeast extract (Oxoid, Basingstoke, UK) and 0.4 % glucose (Fisher Scientific, Loughborough, UK) flasks were inoculated with 2 x 10^8 CFU and incubated at 30M-0C on an orbital shaker at 150 rpm. Growth was monitored spectrophotometrically. Burkholderia lata strains evaluated were: strain 383 (LMG22485) and strain 383-CMIT (a spontaneous mutant with increased tolerance to a commercially available blend of 3:1 methylisothiazolinone & chloromethylisothiazolinone preservatives). Sub-inhibitory concentrations of preservatives added at the time of inoculation were: 0.003% dimethylol dimethyl hydantoin (DDH, Lonza Ltd, Basal, Switzerland) or 0.01 % methylisothiazolinone/chloromethylisothiazolinone (MIT/CMIT, Romm & Haas, Coventry, UK). Only B. lata strain 383 was cultured with dimethylol dimethyl hydantoin. Strain 383 cultivated without preservatives was used as control condition.<br>Cultures were harvested at mid-exponential growth phase (optical density of 0.5, 2 x 10^8 CFU), promptly aliquoted into a microcentrifuge tube and immediately snap-cooled in liquid nitrogen before centrifuging at 20.000 x g at 4M-:C for 1 min. Pellets were immediately frozen at -80M-:C.<br>
Project description:MetQ gene of Streptococcus suis serotype 2 deletion strain has attenuated antiphagocytosis. However,the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope labeling by amino acids in cell culture (SILAC) based liquid chromatography-mass spectrometry (LC-MS) and subsequent bioinformatics analysis was used to determine differentially expressed proteins of RAW264.7 cells infected with △MetQ and ZY05719, aimed at elucidating the mechanism of antiphagocytosis and innate immunity of macrophages infected by Streptococcus suis.