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Transcription profiling of mouse embyonic fibroblasts from wild type and Elavl1knock-out embryos

ABSTRACT: Mouse embryonic fibroblasts (MEF) were isolated via enzymatic dissociation of 3 E14.5 embrya bearing epiblast specific inactivation of the murine Elavl1 locus as 3 littermate controls. Individual Fibroblast cultures were maintained in complete DMEM medium containing 10%FBS at 370C with 5%CO2. Cultures were passaged for 3 times and harvested for RNA isolation when they reached 60-70% conflunecy.

ORGANISM(S): Mus musculus

SUBMITTER: Vaggelis Harokopos

PROVIDER: E-MEXP-1785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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