Chromatin immunoprecipitation of fission yeast to identify topoisomerase I and II activity

ABSTRACT: Eukaryotic topoisomerase I and II relax DNA and are key components in the processes of DNA replication, transcription and genome stability. It is not clear, however, how their activity controls epigenetic states across an entire eukaryotic genome. Using the fission yeast model Schizosaccharomyces pombe, we investigate genome-wide how topoisomerases affect chromatin formation through nucleosome occupancy and regulate transcription. We show that topoisomerase activity is required for nucleosome turnover at promoter regions, affecting epigenetic gene regulatory states, and for effective termination of transcription.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Mickael DURAND-DUBIEF 

PROVIDER: E-MEXP-1850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress