Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Populus x canescens fine roots inoculated with Paxillus involutus in response to salt stress

ABSTRACT: Populus x canescens was inoculated with Paxillus involutus and grown in a climate chamber for 13 weeks. Afterwards, plants were salt stressed for 18 additional days with 150 mM NaCl in the nutrient solution. This resulted in 4 different treatments: no mycorrhiza
control (NC), mycorrhiza/control (MC), no mycorrhiza/salt (NS), mycorrhiza/salt (MS).

ORGANISM(S): Populus tremula x Populus alba

SUBMITTER: Dennis Janz

PROVIDER: E-MEXP-1874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Upgrading root physiology for stress tolerance by ectomycorrhizas: insights from metabolite and transcriptional profiling into reprogramming for stress anticipation.

Luo Zhi-Bin ZB Janz Dennis D Jiang Xiangning X Göbel Cornelia C Wildhagen Henning H Tan Yupeng Y Rennenberg Heinz H Feussner Ivo I Polle Andrea A

Plant physiology 20091007 4

Ectomycorrhizas (EMs) alleviate stress tolerance of host plants, but the underlying molecular mechanisms are unknown. To elucidate the basis of EM-induced physiological changes and their involvement in stress adaptation, we investigated metabolic and transcriptional profiles in EM and non-EM roots of gray poplar (Populus x canescens) in the presence and absence of osmotic stress imposed by excess salinity. Colonization with the ectomycorrhizal fungus Paxillus involutus increased root cell volume ...[more]

PMID: 19812185

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Project description:Salt stress is a primary cause of crop losses worldwide, and it has been the subject of intense investigation to unravel the complex mechanisms responsible for salinity tolerance. MicroRNA is implicated in many developmental processes and in responses to various abiotic stresses, playing pivotal roles in plant adaptation. Deep sequencing technology was chosen to determine the small RNA transcriptome of Saccharum sp cultivars grown on saline conditions. We constructed four small RNAs libraries prepared from plants grown on hydroponic culture submitted to 170mM NaCl and harvested after 1h, 6hs and 24hs. Each library was sequenced individually and together generated more than 50 million short reads. Ninety-eight conserved miRNAs and 33 miRNAs* were identified by bioinformatics. Several of the microRNA showed considerable differences of expression in the four libraries. To confirm the results of the bioinformatics-based analysis, we studied the expression of the 10 most abundant miRNAs and 1 miRNA* in plants treated with 170mM and with a severe treatment of 340mM NaCl. The results showed that 11 selected miRNAs had higher expression in samples treated with severe salt treatment compared to the mild one. We also investigated the regulation of the same miRNAs in shoots of four cultivars grown on soil treated with 170mM NaCl. Cultivars could be grouped according to miRNAs expression in response to salt stress. Furthermore, the majority of the predicted target genes had an inverse regulation with their correspondent microRNAs. The targets encode a wide range of proteins, including transcription factors, metabolic enzymes and genes involved in hormone signaling pathways of, probably assisting the plants to develop tolerance. Our work provides insights into the regulatory functions of miRNAs, thereby expanding our knowledge on potential salt-stressed regulated genes. Screenning of sRNA transcriptome of sugarcane plants infected with Acidovorax avenae subsp avenae after seven days

Dataset Information

Transcription profiling of Populus x canescens fine roots inoculated with Paxillus involutus in response to salt stress

Publications

Upgrading root physiology for stress tolerance by ectomycorrhizas: insights from metabolite and transcriptional profiling into reprogramming for stress anticipation.

Similar Datasets

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