Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of E. coli wild type and ycgF::kan cells treated with blue-light irradiation for 3 hours at 16oC


ABSTRACT: Wildtype and ycgF::kan cells were grown in LB medium at 37°C to an OD(578 nm) of 0,7. Then the cultures were shifted to 16°C and blue-light irradiated for 3 hours.

ORGANISM(S): Escherichia coli

SUBMITTER: Natalia Tschowri 

PROVIDER: E-MEXP-1877 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli.

Tschowri Natalia N   Busse Susan S   Hengge Regine R  

Genes & development 20090201 4


The blue light using FAD (BLUF)-EAL protein YcgF is a known blue-light sensor of Escherichia coli, but its direct regulatory output and physiological function have remained unknown. Here, we demonstrate that unlike other EAL domain proteins, YcgF does not degrade the signaling molecule c-di-GMP, but directly binds to and releases the MerR-like repressor YcgE from its operator DNA upon blue-light irradiation. As a consequence, a distinct regulon of eight small proteins (of 71-126 amino acids) is  ...[more]

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