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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Listeria monocytogenes after infection into murine macrophages

ABSTRACT: P388D1 murine macrophages were cultured in 85 mm tissue culture plates to about semi confluency. L. monocytogenes serotypes (1/2a EGD-e, 4a L99, 4b CLIP80459 and 4b F2365) were infected to the P388D1 cell monolayer at a MOI of 100 per eukaryotic cell. Infection was carried for 45 min and followed by addition of fresh medium containing 20 M-5g/ml gentamicin. The medium of the plates (containing 20 M-5g/ml gentamicin) infected with L. monocytogenes serotypes were replaced after 2 h post infection with fresh medium containing 50 M-5g/ml gentamicin. At each step the plates were washed extensively with 1x PBS. Incubation of the bacterial tissue culture plates was carried out in a humidified incubator for up to 4 h post infection.

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Torsten Hain

PROVIDER: E-MEXP-1947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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