Transcription profiling of Arabidopsis to assess spiking

ABSTRACT: Transcript profiling is crucial to study biological systems and various platforms have been implemented to survey mRNAs at the genome scale. We have assessed the characteristics of the CATMA microarray designed for Arabidopsis thaliana transcriptome analysis, and compared it with two commercial platforms from Agilent and Affymetrix. The CATMA array consists of gene-specific sequence tags of 150 to 500 base pairs, the Agilent (Arabidopsis 2) array of 60mer oligonucleotides, and the Affymetrix gene chip (ATH1) of 25mer oligonucleotide sets. We have matched each probe repertoire with the Arabidopsis genome annotation (TIGR release 5.0) and determined the correspondence between them. Array performance was analyzed by hybridization with labeled target derived from eight RNA samples made of shoo

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Paul Van Hummelen 

PROVIDER: E-MEXP-197 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress