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Transcription profiling of Arabidopsis CYP98A3 knockout mutant

ABSTRACT: TDNA insertion mutants in CYP98A3 were produced by K. Feldmann, and studied through transcriptomic analysis against the wild type WS (specific insertion was checked by PCR and segregation analysis). Characterization of knockout mutants for P450s can be very helpful to fully understand the function of the disrupted gene, and is expected to generate information about the molecular basis of mutants phenotype.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Caroline Proux

PROVIDER: E-MEXP-198 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth.

Abdulrazzak Nawroz N Pollet Brigitte B Ehlting Jürgen J Larsen Kim K Asnaghi Carole C Ronseau Sebastien S Proux Caroline C Erhardt Mathieu M Seltzer Virginie V Renou Jean-Pierre JP Ullmann Pascaline P Pauly Markus M Lapierre Catherine C Werck-Reichhart Danièle D

Plant physiology 20051223 1

Cytochromes P450 monooxygenases from the CYP98 family catalyze the meta-hydroxylation step in the phenylpropanoid biosynthetic pathway. The ref8 Arabidopsis (Arabidopsis thaliana) mutant, with a point mutation in the CYP98A3 gene, was previously described to show developmental defects, changes in lignin composition, and lack of soluble sinapoyl esters. We isolated a T-DNA insertion mutant in CYP98A3 and show that this mutation leads to a more drastic inhibition of plant development and inhibitio ...[more]

PMID: 16377748

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