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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Trypanosoma brucei DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours

ABSTRACT: Induction of the expression of the DHH1 DEAD:DQAD mutant from pLEW100 for 24 hours (thus, M-1 24 hours
Tetracycline). Uninduced cells were used as control.

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Rafael Queiroz

PROVIDER: E-MEXP-2026 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The RNA helicase DHH1 is central to the correct expression of many developmentally regulated mRNAs in trypanosomes.

Kramer Susanne S Queiroz Rafael R Ellis Louise L Hoheisel Jörg D JD Clayton Christine C Carrington Mark M

Journal of cell science 20100202 Pt 5

In trypanosomes, the predominant mechanisms of regulation of gene expression are post-transcriptional. The DEAD-box RNA helicase DHH1 was identified in a screen for gene products that are necessary for the instability of the GPI-PLC mRNA in insect-stage trypanosomes. Expression of an ATPase-deficient dhh1 mutant caused a rapid growth arrest associated with a decrease in polysomes, an increase in P-bodies and a slight decrease in average mRNA levels. However, the effect of dhh1 mutant expression ...[more]

PMID: 20124414

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