Transcription profiling of cat FIV infected lymphocytes at 1, 2, 4, and 24hrs post infection

ABSTRACT: Gene Expression Changes in Lymphocytes at Various Times Post-Feline Immunodeficiency Virus Infection:

Lymphocytes were cultured for 3 to 5 days with hrIL-2 and Con-A, counted, and prepared at a density of 4.0 x 10e6 cells/ml of media. Approximately 10e7 cells were seeded into eight separate 50 ml centrifuge tubes, and cells were treated with polybrene for 1 hour at 37oC.
The cells were washed and infected with 16 TCID50 of USgaB01 for 2 hours, or mock infected with "spent" media. The cells were washed, re-suspended in 10 ml of fresh media, and aliquots were cultured for 1, 2, 4, and 24 hours. Total RNA was isolated at each time
point for microarray analysis. Individual aliquots of lymphocytes were infected on 3 separate
occasions.

ORGANISM(S): Felis catus

SUBMITTER: Ryan J Dowling 

PROVIDER: E-MEXP-204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress