Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human thyroid papillary carcinoma cells treated with TGF-b or untreated

ABSTRACT: We used primary culture of Human thyroid papillary carcinoma to perform the experiment. We make two group one without TGF-b treatment and another with 2nanogr of TGF-b for 4 days. The RNA was extracted and the samples were processed for the array assay.

ORGANISM(S): Homo sapiens

SUBMITTER: Clara V. Alvarez

PROVIDER: E-MEXP-2045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of normal, tumor and pure stromal tissue samples from patients with prostate adenocarcinoma, together with 4 cell lines

Project description:We have used normal, tumoral and pure stromal whole tissue samples, and cells lines in order to identify new markers of prostate cancer. The normal, tumoral and stromal tissue were obtained from patients diagnosed with prostate adenocarcinoma.

2008-11-12 | E-MEXP-1331 | biostudies-arrayexpress

Transcription profiling of white clover plants following infection by white clover mosaic virus

Project description:White clover mosaic virus (WCMV) is a major pathogen of white clover (Trifolium repens L.), with significant effects on yield and persistence. Due to the absence of natural sources of WCMV resistance a transgenic strategy has been employed to produce plants constitutively expressing WCMV replicase gene derivatives, designed to inhibit the propagation of WCMV through an RNA silencing mechanism. A 12,000 feature oligonucleotide microarray has been used to identify global changes in host plant, in addition to virus genome-encoded gene expression associated with WCMV infection in non-transgenic and transgenic WCMV-resistant white clover. Pairwise comparison between the transcriptome of mock-inoculated non-transgenic and WCMV-inoculated transgenic plants provides clear evidence for substantial equivalence between these two genotype/treatments, and demonstrate the efficacy of the transgenic strategy. WCMV- inoculated non-transgenic plants exhibit elevated abundance of many virus-encoded, and host immune response-specific transcripts compared to the transgenic resistant plants or mock-inoculated non-transgenic plants. By contrast, relative to inoculated sensitive plants, the majority of significantly up-regulated genes in mock-inoculated non-transgenic plants or WCMV-inoculated transgenic plants are markers of healthy cellular function. These results, and the occurrence of levels of WCMV-encoded transcripts in inoculated transgenic plants equivalent to those in virus-free plants, confirm the validity of the transgenic RNA silencing approach.

2010-06-30 | E-MEXP-1688 | biostudies-arrayexpress

Transcription profiling of mouse wild type or Batf deficient T cells cultured under various combinations of IL-6 orTGF-beta

Project description:Wild type and Batf deficient T cells were stimulated under various combinations of IL-6 or TGF-beta.

2009-06-01 | E-MEXP-2152 | biostudies-arrayexpress

Transcription profiling by array of mouse Col8a1-Col8a2 deficient and wild type mesangial cells stimulated with TGF-beta1

Project description:Col8a1/Col8a2-deficient mesangial cells and wildtype cells are treated with TGFbeta-1 for 24h. This experiment was replicated once.

2011-01-01 | E-MEXP-2658 | biostudies-arrayexpress

Transcription profiling by array of human gastric cancer cells treated with suberoylanilide hydroxamic acid (SAHA)

Project description:Gastric cancer cell line AGS was treated with suberoylanilide hydroxamic acid (SAHA) for 24 hours. Microarray analysis was done to explore the differentially expressed genes betweenSAHA treated and control cells.

2011-05-01 | E-MEXP-2695 | biostudies-arrayexpress

Transcription profiling by array of mouse BALB/c3T3 cell lines exposed to low or high irradiation doses

Project description:We used the BALB/c3T3 cell line model to correlate citotoxicity and transformation endpoints to expression gene profiles after IR exposure. Citotoxicity and Cell Transformation Assay (CTA) were performed 20h after IR exposure by using BALB/c3T3 cells. At the same time, RNAs of exposed cells to a low dose (0.25Gy) and to a high dose (3Gy) were extracted for microarray experiments.

2010-12-15 | E-MEXP-2830 | biostudies-arrayexpress

microRNA Expression in HCC Tissues and Cells

Project description:Cell Lines, Cultures and HCC Tumor Tissues. Malignant and non-malignant hepatocyte cell lines were obtained from the American Type Culture Collection (Manassas, VA) and cultured as recommended by the supplier. Total RNA from three paires of normal human liver tissue and from hepatocellular carcinoma was obtained from BioChain Institute, Inc. (Hayward, CA). Isolation of MicroRNA. Total RNA was obtained from cell lines and tissue samples using the Totally RNA isolation kit (Ambion, Austin, TX). The miRNA fraction was obtained by flashPAGE purification using the flashPAGEM-^Y Fractionator System (Ambion, Austin, TX). The size of the microRNA fractions were confirmed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Palo Alto, CA).

2011-08-03 | E-MEXP-1125 | biostudies-arrayexpress

Transcription profiling of human CD133 and CD34 positive hematopoietic stem cells imuno-magnetically isolated from bone marrow and umbilical cord blood

Project description:Expression Profiling of CD133 and CD34 positive Hematopoietic Stem Cells imuno-magnetically isolated from Bone Marrow and Umbilical Cord Blood

2009-07-31 | E-MEXP-1890 | biostudies-arrayexpress

Transcription profiling of mouse erythroblasts during erythroid differentiation

Project description:The I/11 and R10 erythroid progenitor cell line was cultivated in serum free medium (StemPro34) supplemented with Epo (0,5U/ml), SCF (100ng/ml) and dexamethasone (10-6M). To identify genes specifically regulated by Epo/SCF-induced polysome recruitment, cells were factor deprived for 4h and subsequently treated for 2h with 5U/ml Epo and 200ng/ml SCF or left untreated. Subsequently we isolated both total and polysome bound mRNA from each condition, which was hybridised to oligonucleotide arrays (Affymetrix). Analysis of data with Rosetta Resolver allowed to identify and compare Epo/SCF induced gene expression in total and polysome bound RNA. To assess gene expression during erythroid differentiation, cells were induced to differentiate in presence of 5U/ml Epo and 0.5mg/ml Fe-loaded transferrin. Polysome bound mRNA was isolated from cells proliferating in presence of Epo, SCF and dexamethasone (renewal conditions), and from cells induced to differentiate for 48h or 60h.

2008-07-11 | E-MEXP-1689 | biostudies-arrayexpress

Transcription profiling by array of human adenocarcinoma in situ (AIS) of the lung and early invasive adenocarcinoma (EIA) samples

Project description:Adenocarcinoma in situ (AIS) of the lung has an extremely favorable prognosis, with a 5-year survival rate of 100%. However, early invasive adenocarcinoma (EIA) often has a fatal outcome. In this study, we compared the expression profiles of AIS with those of EIA showing a fatal outcome, and screened the differentially expressed genes by cDNA microarray.

2010-10-10 | E-MEXP-2825 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data