Unknown,Transcriptomics,Genomics,Proteomics

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Tiling path analysis of a library of transposon mutants generated in Salmonella enterica serovar Typhimurium strain SL1344 before and after infection in mice to identify mutants attenuated in vivo

ABSTRACT: A library of transposon mutants was generated in Salmonella enterica serovar Typhimurium strain SL1344 using custom Tn5 and Mu transposons with outward-facing T7 and SP6 promoters. The library was grown up in vitro, and used as the input pool for mouse infection experiments. An in vivo output pool was obtained from the mouse livers 2 days post-infection.

Genomic DNA was extracted from the input and output pools and digested with the restriction enzyme RsaI. Cy5-labelled RNA run-offs were produced seperately from the T7 and SP6 promoters and hybridised to a tiling microarray along with the Cy3-labelled in vitro transcription products from an untransposed SL1344 wild type control. Analysis of the microarray data allows the location of the transposon inserts to be determined. Mutants that are present in the input pool but that are absent or less prevalent in the output pools are inferred to be attenuated in vivo.

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Roy Chaudhuri

PROVIDER: E-MEXP-2076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dataset Information

Tiling path analysis of a library of transposon mutants generated in Salmonella enterica serovar Typhimurium strain SL1344 before and after infection in mice to identify mutants attenuated in vivo

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