Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human intestinal epithilium in patients suffering from inflammatory bowel disease.

ABSTRACT: Identification of significant regulated genes in the intestinal epithelium (ileum and colon)
in patients suffering from inflammatory bowel disease (normal vs. activ and non-activ
Crohn's disease and Ulcerative Colitis). This experiment is an extension of experiment E-MEXP-1225. We added 12 new hybridizations prepared according to amplification, hybridization and normalization protocols described in E-MEXP-1225.

ORGANISM(S): Homo sapiens

SUBMITTER: Benjamin Funke

PROVIDER: E-MEXP-2083 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in Crohn's disease.

Funke Benjamin B Lasitschka Felix F Roth Wilfried W Penzel Roland R Meuer Stefan S Saile Maria M Gretz Norbert N Sido Bernd B Schirmacher Peter P Autschbach Frank F

Inflammatory bowel diseases 20110106 9

<h4>Background</h4>A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD).<h4>Methods</h4>The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expres ...[more]

PMID: 21830273

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Project description:FOXO transcription factors control cellular formation of reactive oxygen species (ROS), which critically contribute to cell survival and cell death in neuroblastoma. Here, we report that C10orf10, also named M-bM-^@M-^\Decidual Protein induced by Progesterone (DEPP)M-bM-^@M-^], is a direct transcriptional target of FOXO3 in human neuroblastoma. As FOXO3-mediated apoptosis involves a biphasic ROS accumulation, we analyzed cellular ROS levels in DEPP-knockdown cells by live-cell imaging. Knockdown of DEPP prevented the primary and secondary ROS accumulation during FOXO3 activation and attenuates FOXO3-induced apoptosis, whereas its overexpression raises cellular ROS levels and sensitizes to cell death. In neuronal cells, cellular steady state ROS are mainly detoxified in peroxisomes by the enzyme CAT/catalase. As DEPP contains a peroxisomal-targeting-signal-type-2 (PTS2) sequence at its N-terminus that enables protein import into peroxisomes, we analyzed the effect of DEPP on peroxisomal function by measuring the catalase enzyme activity. Catalase activity was reduced by conditional DEPP overexpression and significantly increased in DEPP-knockdown cells. Using live cell imaging and fluorescent peroxisomal and mitochondrial probes we demonstrate that DEPP localizes to peroxisomes and mitochondria in neuroblastoma cells. The combined data indicate that DEPP reduces peroxisomal activity and thereby impairs the cellular ROS detoxification capacity and contributes to death sensitization. SH-EP, NB15 neuroblastoma cells and CCRF-CEM-C7H2 acute lymphoblastic leukemia cells were infected with the retrovirus plasmid pLIB-FOXO3(A3)-Ertm-iresNeo. Gene expression measures of samples with activated FOXO3 transcription factor (3h OHT treated) have been compared to untreated samples (0h time point). To rule out gene regulations by estrogen samples treated for 3 hours with tamoxifen have been compared to the untreated samples. Only genes that were more than two-fold regulated in the first, but not in the second comparison were defined to be FOXO3 regulated.

Dataset Information

Transcription profiling of human intestinal epithilium in patients suffering from inflammatory bowel disease.

Publications

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in Crohn's disease.

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