Transcription profiling of mouse embryonic stem cells with a doxycycline-inducible Xist cDNA transgene introduced into chromosome 3

ABSTRACT: A doxycycline-inducible Xist cDNA transgene was introduced into mouse chromosome 3 at approximately 99.79Mb (NCBI Build m36). Insertion of the transgene was homozygous, so only one copy of chromosome 3 carried the transgene, and the other chromosome 3 was wild-type. Upon doxycycline induction, the transgene is expressed, producing Xist transgene RNA which localises to autosomal material in cis, leading to silencing of genes adjacent to the transgene. Due to the homozygous nature of transgene insertion and the cis nature of silencing, for any silenced gene, the maximum possible downregulation in expression levels was 50%.



To quantify the changes in the levels of gene expression in response to Xist transgene expression, total RNA was extracted from undifferentiated and diff

ORGANISM(S): Mus musculus

SUBMITTER: Amy Tang 

PROVIDER: E-MEXP-2086 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress