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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wounds from ovariectomized MIF null mice and controls to investigate the role of MIF during wound healing using BALB/C MIF null mice

ABSTRACT: The aim of this experiment was to investigate the role of MIF during wound healing using BALB/C MIF null mice and in the context of reduced estrogen-associated impaired healing using ovariectomized mice (a mouse model of age-associated delayed healing). Ageing is associated with delayed cutaneous wound healing resulting from reduced estrogen levels. Macrophage migration inhibitory factor (MIF - NCBI RefSeq: NM_010798) is thought to mediate the effects of estrogen on wound healing. Gene expression was compared between wounds from ovariectomized MIF null mice and controls.

ORGANISM(S): Mus musculus

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Macrophage migration inhibitory factor: a central regulator of wound healing.

Hardman Matthew J MJ Waite Alexander A Zeef Leo L Burow Matthew M Nakayama Toshinori T Ashcroft Gillian S GS

The American journal of pathology 20051201 6

Age-associated differences in estrogen levels critically modify the cutaneous wound healing response. Using a microarray-based approach, we profiled changes in gene expression within the wounds of mice that were wild type or null for the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) in the presence or absence of estrogen. This experimental design identified more than 600 differentially expressed genes and established MIF as a key player in the wound healing process, regu ...[more]

PMID: 16314470

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