Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human mesenchymal stem cell osteogenic differentiation

ABSTRACT: full human genome expression microarrays were used to analysis of gene expression profiles upon the induction of MSC osteogenic differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Jiang Tan

PROVIDER: E-MEXP-2131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Comparative genomic hybridization of Lactobacillus sakei strain 23K and non-sequenced strains

Project description:CGH of L. sakei strain 23K vs non-sequenced strains

2009-09-01 | E-MEXP-1639 | biostudies-arrayexpress

Comparative genomic hybridization of Lactobacillus sakei strains

Project description:CGH of L. sakei strains from various origins.

2009-12-17 | E-MEXP-1969 | biostudies-arrayexpress

A genomic approach to bud mutation in sweet orange (Citrus sinensis L. Osbeck) fruit

Project description:Bud mutations arise often in citrus. The selection of mutants is one of the most important breeding methods in citrus. However, the molecular bases of bud mutation have rarely been studied. To identify the potential important or novel genes involved in bud mutation, different transcriptomic techniques combing suppression subtractive hybridization (SSH) and microarray were performed between a lycopene accumulated mutant, âHong Anliuâ sweet orange (Citrus sinensis L. Osbeck), and its wild-type during fruit maturation. Microarray analysis revealed that differentially expressed clones are extensively coordinated with the initiation of lycopene accumulation. After sequencing of the differentially expressed clones, a total of 267 non-redundant transcripts were obtained, 182 (68.2%) of which share homology (E-value â¤ 1Ã10-10) with known gene products or known protein domains. A list of candidate genes which involved in cellular metabolic process, primary metabolic process, localization, macromolecular metabolic process was obtained. Out of these genes, 12 share homology with previously described signal transduction or transcription factors, suggesting complex regulatory control. These results demonstrate profound effect on gene expression of bud mutation in citrus fruits and provide new insights into the molecular basis of bud mutation. Keywords: bud mutation, candidate genes, Citrus, cNDA microarray, suppression subtractive hybridization (SSH) Fruits from the mutant and its wild type were collected at five time points from August to December. Total RNA extracted from the mutant was hybridized to the array together with RNA from the wild type. Each hybridization was performed in duplicate by dye swap.

2009-03-09 | E-GEOD-10729 | biostudies-arrayexpress

Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

Project description:Seedlessness is an important agronomic trait for citrus, and male sterility (MS) is one main cause of seedless citrus fruit. However, the molecular mechanism of citrus seedlessness remained not well explored. An integrative strategy combining SSH (suppression subtractive hybridization) library with cDNA microarray was employed to study the underlying mechanism of seedlessness of a Ponkan mandarin seedless mutant (Citrus reticulate Blanco). Screening with custom microarray, a total of 279 differentially expressed clones were identified, and 133 unigenes (43 contigs and 90 singletons) were obtained after sequencing. Gene Ontology (GO) distribution showed that the majority of differential genes involved in metabolic process, responded to stimulus and functioned as DNA/RNA binding, catalytic activity and oxidoreductase activity. Expression dynamics of some candidate clones revealed a gene encoding male sterility protein 2 was highly up-regulated, while several transcription factors (TFs) such as AP2/EREBP, MYB, WRKY, NAC and C2C2-GATA zinc-finger domain TFs were specifically down-regulated in the seedless mutant compared with the wild type. Our research highlighted some candidate pathways that participated in the citrus male gametophyte development and could be beneficial for seedless citrus breeding in the future. Flower buds from the seedless citrus mutant and its wild type were collected at four developmental stages: quaring stage (SF, about 20 DBF), medium bud stage (MF, about 10 DBF), flowers at full bloom stage (BF) and young ovaries of 2-3 days after flowering (OV). Total RNA extracted from the mutant and the wild type was hybridized to the array. The hybridization was performed in duplicate by dye swap.

2012-05-21 | E-GEOD-38094 | biostudies-arrayexpress

Comparative genomic hybridization of non-sequenced strains of S. aureus against the sequenced Mu50 strain

Project description:CGH of non-sequenced strains of S. aureus against the sequenced Mu50 strain using TIGR v5 array.

2009-12-31 | E-MEXP-2005 | biostudies-arrayexpress

Comparative genomic hybridization of Staphylococcus aureus sequenced strains in order to assess method of analysis

Project description:CGH of sequenced strains in order to assess method of analysis

2009-12-31 | E-MEXP-2007 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella pertussis clinical isoltates circultating in Senegal

Project description:Genomic content of Bordetella pertussis clinical isolates circulating in Senegal

2009-01-08 | E-MEXP-1674 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella pertussis isolates collected in France in 2007 and a reference strain

Project description:Genomic content of Bortelella pertussis clinical isolates collected in 2007 in France using 3 isolates and one reference strain

2009-10-20 | E-MEXP-1871 | biostudies-arrayexpress

Transcription profiling of B. abortus BvrS BvrR from B. abortus 2308

Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)

2010-04-22 | E-MEXP-2564 | biostudies-arrayexpress

Transcription profiling of Brucella abortus using an ORFeome-based Brucella whole-genome DNA microarray

Project description:A direct analysis of the global transcriptional profile of B. abortus cells grown in laboratory conditions was made. Thus, three independent biological replicas were made, and following the RNA isolation, samples were reverse transcribed in aRNA and labelled with Cy3 fluorescent dye. The use of a single dye labelling approach allowed us an estimation of the genes more abundantly expressed in Brucella under laboratory conditions. The evaluation experiment was composed of three slides yielding six measurements per gene (representing three biological replicates, since each gene is present twice on each slide) to confirm the reproducibility of the gene expression data. <br>The complete dataset was sorted by the normalized and corrected fluorescence intensity values.

2008-11-27 | E-MEXP-1887 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data