ABSTRACT: PDR mutants , strain YYA100 (pdr1?::KanMx6, pdr3?::His3Mx6) from Mol Biol Cell. 2007 December; 18(12): 49324944, was grown to OD 1-1.5 in YEPD and hybridyzed to an expression array in one channel with a wild-type strain reference RNA on the other channel
Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.
Project description:Identification of RNAs bound to Whi3 and Whi4. Mock experiment and several other proteins (Dpm1 and Sfb3) were used as controls. TAP versions of the proteins were used for affinity purification. RNAs retained in the columns were identified by microarray hybridizations.