Transcription profiling of human irradiated MOLT4 cells to measure measure of degradation rates by actinomycin D

ABSTRACT: MOLT4 cells in log phase (1x106 cells/ml) were irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a 137Cs ?-irradiator. After 4 hours, actinomycin D (5 ?M) was added to block transcription. Aliquots of culture were removed at hourly intervals and RNA was extracted (Trizol; Invitrogen). RNA and cRNA quantity and quality was determined by Nanodrop spectrophotometer and Bioanalyser 2100 (Agilent). Transcript levels were determined at different time points using Affymetrix Human U133A microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Efterpi Papouli 

PROVIDER: E-MEXP-2179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress