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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a ramA over-expressing strain

ABSTRACT: The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic ramA gene knockout mutant which contained ramA upon an IPTG-inducible over-expression plasmid.

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Andrew Bailey

PROVIDER: E-MEXP-2195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RamA, a member of the AraC/XylS family, influences both virulence and efflux in Salmonella enterica serovar Typhimurium.

Bailey Andrew M AM Ivens Al A Kingsley Rob R Cottell Jennifer L JL Wain John J Piddock Laura J V LJ

Journal of bacteriology 20100115 6

The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 lacking a functional ramA or ramR or with plasmid-mediated high-level overexpression of ramA were compared to those of the wild-type parental strain. Inactivation of ramA led to increased expression of 14 SPI-1 genes and decreased expression of three SPI-2 genes, and it altered expression of ribosomal biosynthetic genes and several amino acid biosynthetic pathways. Furthermore, disruption of ramA led to decreased survival withi ...[more]

PMID: 20081028

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Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 marA knockout

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Minimal vs LB media comparison

Project description:The type of bacterial culture medium is an important consideration during design of any experimental protocol. The aim of this study was to understand the impact of medium choice on bacterial gene expression and physiology by comparing the transcriptome of Salmonella enterica SL1344 after growth in the widely used LB broth or the rationally designed MOPS minimal medium. Transcriptomics showed that after growth in MOPS minimal media, compared to LB, there was increased expression of 42 genes involved in amino acid synthesis and 23 genes coding for ABC transporters. Seven flagellar genes had decreased expression after growth in MOPS minimal medium and this correlated with a decreased motility. In both MOPS minimal medium and MEM expression of genes from SPI-2 was increased and the adhesion of S. Typhimurium to intestinal epithelial cells was higher compared to the levels after growth in LB. However, SL1344 invasion was not significantly altered by growth in either MOPs minimal media or MEM. Expression of SPI-2 was also measured using chromosomal GFP reporter fusions followed by flow cytometry which showed, for the first time, that the reduction in SPI-2 transcript after growth in different media related to a reduction in the proportion of the bacterial population expressing SPI-2. These data highlight the profound differences in the global transcriptome after in vitro growth in different media and show that choice of medium should be considered carefully during experimental design, particularly when virulence related phenotypes are being measured.

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