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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Schistosoma mansoni adult male and female worms isolated from mice 7-weeks post-infection

ABSTRACT: Adult male and female Schistosoma mansoni worms were isolated from mice 7-weeks post-infection. Male material (Cy5) and female material (Cy3) were compared in a direct bimodal comparison. Five independent biological batches were used. Addtional dye-swap experiments male (Cy3) and female (Cy5) were performed using pooled samples of total RNA.

ORGANISM(S): Schistosoma mansoni

SUBMITTER: Tom Freeman

PROVIDER: E-MEXP-223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression.

Fitzpatrick Jennifer M JM Johnston David A DA Williams Gary W GW Williams Debbie J DJ Freeman Tom C TC Dunne David W DW Hoffmann Karl F KF

Molecular and biochemical parasitology 20050501 1

Global profiling transcriptomes of parasitic helminths offers the potential to simultaneously identify co-ordinately expressed genes, novel genetic programs and uniquely utilized metabolic pathways, which together provide an extensive and new resource for vaccine and drug discovery. We have exploited this post-genomic approach to fabricate the first oligonucleotide DNA microarray for gene expression analysis of the parasitic trematode Schistosoma mansoni. A total of 17,329 S. mansoni DNA sequenc ...[more]

PMID: 15811522

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Transcription profiling of Bi-sex male and female Schistosoma mansoni worms isolated from mice 7-weeks post-infection

Project description:Bi-sex male and female Schistosoma mansoni worms were isolated from mice 7-weeks post-infection. Bi-sex male material or female material was compared in a direct bimodal comparison to single-sex male or female material. Two independent biological batches of both bi- and single-sex males were used. One batch of single-sex female material was used in comparisons to three independent biological batches of bi-sex female material. A dye-swap hybridization was performed for each bimodal comparisons in turn.

2006-11-01 | E-MEXP-474 | biostudies-arrayexpress

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2014-12-31 | E-MTAB-2135 | biostudies-arrayexpress

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2010-08-01 | E-GEOD-21878 | biostudies-arrayexpress

BS-90 strain resistance in M-line Biomphalaria glabrata

Project description:Transcriptional profiles ofBS-90 snails sized 8-12mm exposed to E. paraensei (BS-90 are susceptible) and S. mansoni (BS-90 are resistant) and unexposed controls Keywords: Dose response Five individual snails from the treatment group were pooled to make up 3 repliactes. After exposure to the indicated parasite snails were collected and RNA was isolated at 12hours, 1, 2, 4, 8, 16, and 32 days. Three unexposed control groups were also analyzed.

2010-08-01 | E-GEOD-21881 | biostudies-arrayexpress

Size resistance in M-line Biomphalaria glabrata

Project description:Transcriptional profiles of snails sized 12-20mm exposed to E. paraensei and unexposed controls Keywords: Dose response Five individual snails from the treatment group were analyzed at 12hours, 1, 2, 4, 8, and 16 days. Five unexposed control snails were also analyzed.

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