Transcription profiling of mouse 6780 cell line derived from murine T-cell lymphomaTGFbeta dependent gene expression in T-cell lymphomas

ABSTRACT: The cell line "6780" derived from murine T-cell lymphoma was grown in RPMI medium 1640 with 10% FBS/1% penicillin/Streptomycin/1% l-glutamine/50 μM 2-mercaptoethanol. Vector constructs containing a dominant negative mutant of the human TGF-beta receptor type II (pMSCV-TGFBR-IRES-GFP) or an empty vector (pMSCV-IRES-GF) were kindly provided by Martin Eilers Group, IMT, Marburg, Germany. Cells were incubated with retroviruses containing supernatants for 12 h at 32°C in media containing 4 ?g/ml polybrene. Cells then were expanded at 37°C for an additional 48 h, and GFP-expressing cells were purified by flow cytometry on a FACS Vantage (Becton Dickinson).



All animal experiments were performed by following the guidelines from Administrative Panel on Laboratory Animal Care at Stan

ORGANISM(S): Mus musculus

SUBMITTER: Michael Krause 

PROVIDER: E-MEXP-2233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress