Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of livers of adult UPF2 null mice in response to partial hepatechtomy

ABSTRACT: Deletion of the NMD component UPF2 in adult liver and response to partial hepatechtomy. Lethally irradiated Upf2flox/flox and Upf2flox/flox; Mx1Cre animals were transplanted with WT BM at 10-12 weeks of age. 5-6 following transplantion the bone marrow transplanted (BMT) animals were injected 3 times with poly-IC (Days 0, 2, 4), which facilitates Cre-mediated deletion in BMT Upf2flox/flox; Mx1Cre (hitherto UPF2 null) but not in BMT Upf2flox/flox control (hitherto WT) mice. A day 14 mice were subjected to partial hepatechtomy (0 hours) and sacrificed after 36 hours (36 hours)..

ORGANISM(S): Mus musculus

SUBMITTER: Bo Porse

PROVIDER: E-MEXP-2249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

UPF2 is a critical regulator of liver development, function and regeneration.

Thoren Lina A LA Nørgaard Gitte A GA Weischenfeldt Joachim J Waage Johannes J Jakobsen Janus S JS Damgaard Inge I Bergström Frida C FC Blom Anna M AM Borup Rehannah R Bisgaard Hanne Cathrine HC Porse Bo T BT

PloS one 20100719 7

<h4>Background</h4>Nonsense-mediated mRNA decay (NMD) is a post-transcriptional RNA surveillance process that facilitates the recognition and destruction of mRNAs bearing premature terminations codons (PTCs). Such PTC-containing (PTC+) mRNAs may arise from different processes, including erroneous processing and expression of pseudogenes, but also from more regulated events such as alternative splicing coupled NMD (AS-NMD). Thus, the NMD pathway serves both as a silencer of genomic noise and a re ...[more]

PMID: 20657840

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Project description:To investigate the pathological effect of miR-126 on the progression of acute myeloid leukemia (AML) induced by AML1-ETO9a (AE9a), we conducted a series of mouse bone marrow transplantation (BMT) assays with the following groups: AE9a (primary donor cells were wild-type mouse bone marrow progenitor (i.e., lineage negative; Lin-) cells retrovirally transduced with MSCV-PIG-AE9a), AE9a+miR-126 (primary donor cells were wild-type mouse bone marrow progenitor (i.e., Lin-) cells retrovirally transduced with MSCV-PIG-AE9a-miR-126), and miR-126KO+AE9a (primary donor cells were miR-126 knockout mouse bone marrow progenitor (i.e., Lin-) cells retrovirally transduced with MSCV-PIG-AE9a), along with a control group (primary donor cells were wild-type mouse bone marrow progenitor (i.e., Lin-) cells retrovirally transduced with MSCV-PIG empty vector). The control group was only used in the primary and secondary BMT assays, whereas the three leukemic groups including AE9a, AE9a+miR-126 and miR-126KO+AE9a were used in four passages (i.e., primary, secondary, tertiary and quaternary) of BMT assays. Then, gene expression profiling was conducted with bone marrow samples collected from different groups to decipher the molecular mechanisms underlying miR-126 effects on leukemia initiation and progression and maintenance and self-renewal of leukemia stem/initiating cells. A total of 39 mouse bone marrow samples including 36 mouse AML samples with AE9a, AE9a+miR-126, and miR-126KO+AE9a collected from the 1st, 2nd, 3rd and 4th passages of BMT recipient mice (3 mice for each group in each passage), along with 3 normal controls from the 1st passage of BMT, were analyzed by use of Affymetrix GeneChip Mouse Gene 2.0 ST Array (Affymetirx, Santa Clara, CA). For each sample, the CD45.1+ cells (i.e., transplanted donor cells) were sorted with flow cytometry from whole BM cells collected from BMT recipient mice at the end stage. Then total RNA was isolated by use of miRNeasy extraction kit (Qiagen, Valencia, CA).

Dataset Information

Transcription profiling of livers of adult UPF2 null mice in response to partial hepatechtomy

Publications

UPF2 is a critical regulator of liver development, function and regeneration.

Similar Datasets

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