Transcription profiling of Arabidopsis dms4-1, drd1-1 and drd1-6 mutant seedlings against wild-type to identify targets of IWR1-like transcription factor

ABSTRACT: We identified in a forward genetic screen a novel factor required for RNA-directed DNA methylation (IWR1-like transcription factor). The aim of the study was to identify natural targets of this factor and to compare it to targets of drd1 another major key factor required for the RNA silencing process in plants. We performed transcriptome analysis to identify targets of this factor. For this we used whole plants at seedling stage, isolated total RNA and then used the standard Affymetrix pipeline/chemistry. The mutated transcription factor, dms4-1 was compared to its respective wildtype (EDT, Col-O background). For microarray analysis THREE biological replicates were run.

For transcriptome comparison we analyzed another factor required for the process RNA-directed DNA methylation in p

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Bruno Huettel 

PROVIDER: E-MEXP-2251 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress