Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

5 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human endothelial cells UPR signature and concomitant activation of multiple signaling pathways in cells exposed to hGX sPLA2 modified LDL

ABSTRACT: We conducted a global transcriptional profiling using microarray analysis of the response of EC exposed either to native LDL or LDL-X

ORGANISM(S): Homo sapiens

SUBMITTER: Seraya Maouche

PROVIDER: E-MEXP-2256 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Phospholipolyzed LDL induces an inflammatory response in endothelial cells through endoplasmic reticulum stress signaling.

Gora Sarah S Maouche Seraya S Atout Rajai R Wanherdrick Kristell K Lambeau Gérard G Cambien François F Ninio Ewa E Karabina Sonia-Athina SA

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20100429 9

Secreted phospholipases A2 (sPLA2s) are present in atherosclerotic plaques and are now considered novel attractive therapeutic targets and potential biomarkers as they contribute to the development of atherosclerosis through lipoprotein-dependent and independent mechanisms. We have previously shown that hGX-sPLA2-phospholipolyzed LDL (LDL-X) induces proinflammatory responses in human umbilical endothelial cells (HUVECs); here we explore the molecular mechanisms involved. Global transcriptional g ...[more]

PMID: 20430794

Similar Datasets

Transcription profiling of rice wild type and OsSSI2 knock down plants

Project description:Rice genes regulated by OsSSI2 (a fatty acid-desaturase) profiled by comparing between wild-type and OsSSI2-knockdown plants.

2009-03-26 | E-MEXP-1815 | biostudies-arrayexpress

Transcription profiling of rat brain after prolonged administration of Phenytoin

Project description:In order to elucidate the molecular mechanisms of action of Phenytoin, we examined by microarrays the effects of prolonged administration of Phenytoin on gene expression in hippocampus and frontal cortex of Sprague-Dawley rats chronically treated with Phenytoin.

2009-12-31 | E-MEXP-1728 | biostudies-arrayexpress

Transcription profiling of human peripheral blood mononuclear cells from radiation-exposed workers versus control subjects

Project description:We used peripheral blood mononuclear cells to study the gene expression profiles of radiation-exposed workers versus control subjects. Human PBMC were isolated from 56 healthy donors who were working in a local Hospital (Bologna, Italy): 28 individuals were x and g radiation-exposed workers, recruited from the Nuclear Medicine and Cardiovascular Units, while the control group consisted of 28 unexposed subjects, working at the same health care facility, matched for sex, age and smoking habits. The mean dosimeter reading, expressed as accumulated doses, during the entire time of employment (range: 1-32 years), was 19.49±37.59 mSv (mean ± SD). All procedures related to the collection of blood samples were performed with an informed consent and according to the protocol approved by the Institutional Review Board.

2008-11-15 | E-MEXP-1277 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild-type and transgenic plants overexpressing the AtCPK1 gene

Project description:Transcription profiles of wild-type and transgenic Arabidopsis plants overexpressing the AtCPK1 gene

2011-01-07 | E-MEXP-2532 | biostudies-arrayexpress

Transcription profiling of human PC-3 prostate cancer cells expressing shTCEB1 leading to TCEB1 silencing

Project description:Lentivirus mediated RNAi was used to silence TCEB1 expression in PC-3 prostate cancer cell line. Whole human genome microarray was used to study changes in gene expression induced by the silencing.

2008-10-31 | E-MEXP-1627 | biostudies-arrayexpress

Transcription profiling by array of human ST88 cells treated with FTS

Project description:Comparing gene profile of ST88 cells treated with 75µM FTS in 5% serum to those of control

2011-04-01 | E-MEXP-2766 | biostudies-arrayexpress

Transcription profiling of human thyroid follicular adenomas and thyroid papillary carcinomas to discriminate benign and malignant tumors

Project description:Identification of a stable gene expression signature with high classifying potential to discriminate benign (Follicular adenomas) and malignant (papillary carcinomas) thyroid tumors

2010-08-01 | E-MEXP-2335 | biostudies-arrayexpress

Transcription profiling of livers of C57BL/6 mice and LDL receptor deficient mice fed on either low fat or high fat diet to study liver gene expression associated with diet.

Project description:Examination of gene expression profiles from liver of C57BL/6 mice and LDL receptor deficient mice fed on either a low fat diet or a high fat Western-style diet for 12 weeks. Three replicates of each condition analyzed. Keywords = LDL receptor deficiency, high fat diet, atherosclerosis, liver

2007-11-01 | E-GEOD-363 | biostudies-arrayexpress

Presence of ceramidase activity in electronegative LDL

Project description:Electronegative LDL (LDL(-)) is a minor modified fraction of human plasma LDL with several atherogenic properties. Among them, LDL(-) has increased content of bioactive lipid mediators, such as lysophosphatidylcholine (LPC), non-esterified fatty acids (NEFA), ceramide (Cer), and sphingosine (Sph), which are related to the presence in LDL(-) of some phospholipolytic activities, including platelet-activating factor acetylhydrolase (PAF-AH), phospholipase C (PLC) and sphingomyelinase (SMase). However, the activity of these enzymes does not explain the increased content of Sph, which should derive from Cer degradation. In the present study we analyzed the putative presence of a ceramidase (CDase) activity that could explain the increased content of Sph. Thin layer chromatography (TLC) and lipidomic analysis showed that Cer, Sph and NEFA spon-taneously increased in LDL(-) incubated alone at 37ºC, in contrast with native LDL(+). An inhibitor of neutral CDase prevented the formation of Sph and, in contrast, increased the content of Cer in LDL(-). In addition, a fluorescently-labelled Cer (NBD-Cer) was efficiently degraded by LDL(-) to form Sph and NEFA. These observations point to the existence of a CDase activity associated to LDL(-). However, neither proteomic analysis nor western blot detected the presence of a known CDase enzyme. Further studies are warranted to define the origin of the CDase activity detected in LDL(-).

2023-01-09 | PXD036168 | Pride

Transcription profiling by array of human universal RNA spiked with eight arifical transcripts at different copy numbers

Project description:Examine the dose response characteristics of a set of eight artificial transcripts (IVT) when spiked into a Universal Human RNA background at different copy numbers. The experiment was designed to <br>(i) evaluate the technical reproducibility of array data generated on the Agilent Whole Human 44K array with use of a set of external RNA controls <br> (ii) employ two panels of the same external RNA controls, which differed in copy number between panels, in order to simulate normal and disease states. We employed 8 different pools which were comprised of 8 different external RNA standards in a total human reference RNA background. Each different standard was present at a different copy number within any single pool (see file: E-MEXP-2670.additional.zip). Each control was present at a different concentration in each of the 8 pools. Standard microarray analysis was performed on the data and the differences in abundance between external RNA controls determined in terms of fold change differences.

2011-03-30 | E-MEXP-2670 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data