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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human U2OS cell line transfected with IZ-1 and 14 ARF expressing plasmids

ABSTRACT: U2OS cells were stably transfected with an ecotropic receptor expression plasmid. These cells were infected with retroviruses expressing MIZ-1 or MYC and subsequently superinfected with retroviruses expressing p14ARF. Selection was carried out 48h following superinfection and selected cells were harvested within 1 - 2 passages. For each condition around 2.5x10 cells were pooled.
Using the two color Quick-Amp labelling kit (Agilent, 5190-0444) 100ng of total RNA were used for cDNA synthesis, aRNA amplification and labelling according to manufacturer's instructions.
Transcriptional profiling was done on a whole human genome oligo microarray (Agilent, G4112F, 014850) in a 4x44k slide format.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Krause

PROVIDER: E-MEXP-2310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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