Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Arabidopsis srk2cf mutant plants under abscisic acid (ABA) treatment for 0, 1 and 4 hours

ABSTRACT: Microarray experiments were performed using Arabidopsis wild type plants (Col-0) and srk2cf double knockout mutants to investigate functions of two osmotic stress-activated protein kinases, SRK2C and SRK2F. Transcription profiles of wild type and mutants were compared under abscisic acid (ABA) treatment for 0, 1 and 4 h.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Masahide Mizoguchi

PROVIDER: E-MEXP-2378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression.

Mizoguchi Masahide M Umezawa Taishi T Nakashima Kazuo K Kidokoro Satoshi S Takasaki Hironori H Fujita Yasunari Y Yamaguchi-Shinozaki Kazuko K Shinozaki Kazuo K

Plant & cell physiology 20100407 5

The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/A ...[more]

PMID: 20375108

Similar Datasets

Transcriptional profiling by array of Arabidopsis ABA auxotroph to identify early downstream targets of transcription in response to a small dose of abscisic acid (ABA)

Project description:Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added cycloheximide to preferentially obtain immediate targets of ABA addition. We believe that using a sensitized background of an ABA auxotroph would yield a set of genes that are very closely regulated by ABA at the transcriptional level. This data set will be used for network analysis of ABA signaling.

2011-10-30 | E-MEXP-3404 | biostudies-arrayexpress

Transcription profiling of Arabidopsis srk2cf mutant plants under drought stress for 0, 1 and 4 hours

Project description:Microarray experiments were performed using Arabidopsis wild type plants (Col-0) and srk2cf double knockout mutants to investigate functions of two osmotic stress-activated protein kinases, SRK2C and SRK2F. Transcription profiles of wild type and mutants were compared under drought stress for 0, 1 and 4 h.

2009-10-15 | E-MEXP-2377 | biostudies-arrayexpress

CRK36 RNAi ABA treatment

Project description:Genome-wide expression studies by an Arabidopsis 3 Oligo Microarray (Agilent Technologies, Switzerland) were performed using wild-type and CRK36 RNAi transgenic plants growing on plate containing ABA. We collected young seedlings growing on the plate containing 0.5 ?M ABA for 10 days.

2013-08-28 | E-MEXP-3354 | biostudies-arrayexpress

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

Project description:Transcriptional profiling of a DEX-inducible SNRK3.15 seedlings in the presence of ABA.

2012-03-01 | E-MEXP-3397 | biostudies-arrayexpress

Transcription profiling of Arabidopsis areb1 areb2 abf3 treated with ABA 6h

Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with or without 50 µM ABA for 6 h.

2009-12-17 | E-MEXP-2187 | biostudies-arrayexpress

Abscisic acid (ABA) application to Populus × canescens in hydroponics

Project description:4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatment (+ABA) and discontinuous 100 µM ABA treatment (±ABA). ABA was fed to +ABA plants during the whole treatment period of 30 days. ABA was fed to ±ABA plants for three days in two weeks. Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. RNA was extracted from these samples and followed by RNA-sequencing.

2019-01-20 | E-MTAB-7587 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and srk2dei triple mutant plants in response to abscisic acid, salt and dehydration treatments

Project description:Expression profiles of the wild-type Arabidopsis (Col) and srk2dei triple mutant plants in response to ABA, NaCl, and dehydration treatments.<br><br>

2009-12-17 | E-MEXP-2116 | biostudies-arrayexpress

Transcriptome analysis of Arabidopsis thaliana G protein subunit mutants in response to abscisic acid (ABA)

Project description:Heterotrimeric G proteins mediate crucial and diverse signaling pathways in eukaryotes. To gain insights into the regulatory modes of the G protein and the co-regulatory modes of the G protein and the stress hormone abscisic acid (ABA), we generated and analyzed gene expression in G protein subunit single and double mutants of the model plant Arabidopsis thaliana. Through a Boolean modeling approach, our analysis reveals novel modes of heterotrimeric G protein action. Keywords: transcriptome analysis; G protein subunit mutants; abscisic acid (ABA) Microarray data were generated from four genotypes (wild type, gpa1-4 mutant, agb1-2 mutant, agb1-2 gpa1-4 double mutant) with or without ABA treatment. Arabidopsis plants were grown in growth chambers with an 8 hr light/16hr dark. Three hundred Arabidopsis leaves excised from 60-70 five-week-old plants were used as the starting material for each guard cell microarray. Ten mature leaves taken from 3-4 plants grown side-by side with the plants for guard cell isolation were used for each leaf sample. Excised leaf and isolated guard cell samples were treated with ABA (50 μM) or EtOH (solvent control) for 3 hrs. For each type of sample (guard cells or leaves), three independent biological replicates were performed, resulting in a total of 48 microarray hybridizations (2 sample types ´ 4 genotypes ´ two treatments ´3 replicates).

2010-12-06 | E-GEOD-19520 | biostudies-arrayexpress

Shoot and root of barley seeds: Abscisic acid (ABA) treatment

Project description:Transcriptional profiling of barley shoot and root with ABA Time course experiments

2014-04-01 | E-GEOD-41511 | biostudies-arrayexpress

Microarray analysis of an Arabibidopsis mutant srk2dei

Project description:Microarray experiments were performed using Arabidopsis wild type plants (Col-0) and srk2dei triple knockout mutant to investigate the functions of ABA-activated protein kinases, SRK2D/SnRK2.2, SRK2E/OST1 and SRK2I/SnRK2.3. Transcription profiles of wild type and mutants were compared under ABA treatment or dehydration stress for 0 and 90 min. The srk2dei mutant was established by crossing T-DNA insertion mutants provided from Arabidopsis Biological Resource Center.

2013-03-31 | E-MEXP-3713 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data