Project description:S. meliloti genes under the control of RpoE4 were identified by determining the whole genome <br>transcription profile of a strain over-expressing rpoE4 under the control of the arabinose-dependent<br>PBAD promoter of plasmid pMLBAD-rpoE4. A strain containing the empty vector pMLBAD<br>was used as a reference.<br>
Project description:S. meliloti genes under the control of RpoE1 were identified by determining the whole genome <br>transcription profile of a strain over-expressing rpoE1 under the control of the arabinose-dependent<br>PBAD promoter of plasmid pMLBAD-rpoE1. A strain containing the empty vector pMLBAD<br>was used as a reference.
Project description:The Sinorhizobium meliloti rpoE4 regulon was determined by comparing the wildtype and rpoE4 mutant transcriptomes in the presence of 20mM sodium thiosulfate, an RpoE4 activating condition.
Project description:Microarrays were used to identify Y. pestis genes that are differentially regulated under conditions of phoP overexpression. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one overexpressing phoP (KIM10+phoP(delta)/PhoP) and the other not (KIM10+phoP(delta)/MMB67EH), and their gene expression profiles were compared.
Project description:Microarrays were used to identify Y. pestis genes that are transcriptional targets of phoP. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one carrying wild-type phoP (KIM6+) and the other carrying a deletion within phoP (KIM6+phoP(delta)), and their gene expression profiles were compared.
Project description:S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inoculation culture was added to 50 ml of fresh MM. At various times, samples were removed for determining viable cells counts as well as for RNA isolation/preparation (7 and 14 hours). For experiments on plates, 20 ml of MM containing 0.7% (Semisolid) or 1.3% (Solid) agar was dispensed onto each Petri dish and allowed to gel. The plates were air dried at room temperature for 15 min. 0.1 ml of the inoculation culture was plated onto the surface of the plates and allowed to dry for 10 min. The plates were then inverted and incubated at 30ºC.
Project description:1. Comparison of transcripts during growth in pure culture of a wild-type strain compared to a mutant deficient in the transcriptional activator encoded by the gene prhG.<br><br>2. Transcriptome analysis for identification of the prhG regulon by comparison of a prhG overexpressing mutant to the wild type strain.