Transcriptional profiling of human genetically modified and wild type lung cancer cells H1299 Xenografts from mouse.

ABSTRACT: Human H1299 lung cancer cells or clones engineered for tetracycline-inducible expression of wild type IRP2, or the deletion mutant IRP2?73 (lacking a specific insert of 73 amino acids), were injected subcutaneously into nude mice. The induction of IRP2 profoundly stimulated the growth of tumor xenografts, and this response was blunted by addition of tetracycline in the drinking water of the animals, to turnoff the IRP2 transgene. Interestingly, IRP2?73 failed to promote tumor growth above control levels. As expected, xenografts expressing the IRP2 transgene exhibited high levels of transferrin receptor 1 (TfR1); however, the expression of other known IRP targets was not affected. Moreover, these xenografts manifested increased c-MYC levels and ERK1/2 phosphorylation. A microarray analysis

ORGANISM(S): Homo sapiens

DISEASE(S): Xenografts with H1299 cell line

SUBMITTER: Ignat Drozdov 

PROVIDER: E-MEXP-2524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress