Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of cold and phosphlipase treated Arabidopsis tto analyse the effect of Phospholipases C and D

ABSTRACT: The study aims at determining the genes that are induced or repressed by cold in the suspension cells. It also aims at monitoring the effect of inhibitors of PLC (U73122 vs. U73343) and PLD (ethanol vs. tertiary butanol) on cold response at the transcription level. To answer this question we chose a pharmacological approach. We decided to use molecules that are going to inhibit PLC or PLD. For PLC we use U73122 and its inactive analog as a control; for PLD we used a primary alcohol (ethanol) and a tertiary alcohol (tert-butanol) as a control. We therefore extracted RNA after the following treatments:
4 °C
22 °C
4 °C + 0.9% primary-ethanol
4 °C + 0.9% tertiary-butanol
4 °C + 60 micromolaire U73122
4 °C + 60 micromolaire U73343
The exposure at 4 °C was 4 hours.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ludivine Taconnat

PROVIDER: E-MEXP-259 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dataset Information

Transcription profiling of cold and phosphlipase treated Arabidopsis tto analyse the effect of Phospholipases C and D

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