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Transcription profiling of Arabidopsis thaliana seedlings using different Affymetrix RNA labelling protocols

ABSTRACT: Here we compare transcriptomic data generated using Affymetrix one-cycle (standard labelling protocol), two-cycle (small-sample protocol) and IVT-Express protocols with the Affymetrix ATH1 array using Arabidopsis root samples. Results obtained with each protocol are broadly similar. However, we show that there are 35 probe sets (of a total of 22810) that are misrepresented in the two-cycle data sets. Of these, 33 probe sets were classed as mis-amplified when comparisons of two independent publicly available data sets were undertaken.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Wilson

PROVIDER: E-MEXP-2608 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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