Transcription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells

ABSTRACT: BPH-1 prostate epithelial cells when cultured in Matrigel form 3D acini which closely recapitulate the tissue architecture of the prostate. The presence of primary stroma increases epithelial adhesions and causes changes in morphology. To identify the stromal signals which control actin/cadherin dynamics in epithelial cells we performed a microarray analysis on the stroma using Affymetrix U133 plus 2.0 arrays. Primary stroma from 7 different patients were cultured with (PSE) or without (PS) 3D BPH-1 epithelial acini to determine the gene expression changes within the stroma. The RNA from the Primary epithelia and stromal cultures were used at passages 1 or 1-3, respectively.

ORGANISM(S): Homo sapiens

SUBMITTER: Karen Chambers 

PROVIDER: E-MEXP-2657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress