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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells

ABSTRACT: BPH-1 prostate epithelial cells when cultured in Matrigel form 3D acini which closely recapitulate the tissue architecture of the prostate. The presence of primary stroma increases epithelial adhesions and causes changes in morphology. To identify the stromal signals which control actin/cadherin dynamics in epithelial cells we performed a microarray analysis on the stroma using Affymetrix U133 plus 2.0 arrays. Primary stroma from 7 different patients were cultured with (PSE) or without (PS) 3D BPH-1 epithelial acini to determine the gene expression changes within the stroma. The RNA from the Primary epithelia and stromal cultures were used at passages 1 or 1-3, respectively.

ORGANISM(S): Homo sapiens

SUBMITTER: Karen Chambers

PROVIDER: E-MEXP-2657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Stroma regulates increased epithelial lateral cell adhesion in 3D culture: a role for actin/cadherin dynamics.

Chambers Karen F KF Pearson Joanna F JF Aziz Naveed N O'Toole Peter P Garrod David D Lang Shona H SH

PloS one 20110418 4

<h4>Background</h4>Cell shape and tissue architecture are controlled by changes to junctional proteins and the cytoskeleton. How tissues control the dynamics of adhesion and cytoskeletal tension is unclear. We have studied epithelial tissue architecture using 3D culture models and found that adult primary prostate epithelial cells grow into hollow acinus-like spheroids. Importantly, when co-cultured with stroma the epithelia show increased lateral cell adhesions. To investigate this mechanism fu ...[more]

PMID: 21533155

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