Browse
Submit Data
Databases
API
Help

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of yeast cultured in the presence of dendritic cells

ABSTRACT: In order to establish the optimal conditions to study expression of genes of S. cerevisiae upon interaction with dendritic cells, Saccaromyces cerevisiae cells were cultured in presence of dendritic cells for 4 hours.
Dendritic cells were then treated with zymolase, the yeast cells recovered and lysed. RNA from lysed yeast cells was used for a transcription profiling analysis on Agilent arrays comparing internalized yeast with control yeast.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Luca Beltrame

PROVIDER: E-MEXP-2679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Solanum tuberosum tubers growing at different velocities

Project description:X-ray CT was used to determine the growth velocity of individual tubers. RNA was extracted from tubers growing at different velocities and used for microarray hybridisation.

2010-02-08 | E-MEXP-2484 | biostudies-arrayexpress

Transcription profiling of Solanum tuberosum during tuber induction from a stolon tip to a tuber

Project description:The experiment followed transcriptional changes during potato tuber induction from a stolon tip to a tuber. Samples were taken at stage 1, stage 3, stage 4 and stage 5 according to Kloosterman et al., 2005

2010-02-08 | E-MEXP-2482 | biostudies-arrayexpress

Transcription profiling by array of mouse embryo fibroblasts from wild type and sp3 E553D knock in mutants defective for SUMOylation

Project description:Transcription profiling of mouse embryo fibroblasts E13.5, wildtype and Sp3 E553D knockin mutant defect for SUMOylation

2010-12-31 | E-MEXP-2755 | biostudies-arrayexpress

Expression profiling, U2OS, Aurora kinase and p38 inhibitor response

Project description:U-2 OS (human osteosarcoma cell line) were treated with ZM447439 (an aurora kinase inhibitor), SB202190 (a p38 inhibitor) or ZM447439+SB202190 and resulting changes in gene expression were profiled.

2017-07-01 | E-MTAB-1210 | biostudies-arrayexpress

Transcription profiling by array of mouse Srd5a3 mutant embryos

Project description:Transcriptional profiling of e8.5 mouse embryos comparing wt with Srd5a3Gt(betaGeo)703Lex/Gt(betaGeo)703Lex. Goal was to determine the developmental pathway disrupted in the mutant.

2010-07-01 | E-MEXP-2713 | biostudies-arrayexpress

Expression profiling, MDA-MB-231-luc2 response to GW501516

Project description:MDA-MB-231-luc2 cells were treated with the PPARd specific agonist GW501516 and the resulting expression changes profiled.

2013-06-25 | E-MTAB-1262 | biostudies-arrayexpress

Transcription profiling by array of human WPMY-1 cells treated with GW501516 ((a PPARbetadelta agonist) or TFGBeta2

Project description:Microarray analyses of WPMY-1 cells, either untreated (solvent) or treated with GW501516 (0.3 µM), TGFBeta2 (10 ng/ml), or both ligands for 6 h.

2010-07-21 | E-MEXP-2748 | biostudies-arrayexpress

Transcription profiling by array of mouse hippocampus and prefrontal cortex from mouse model of anxiety

Project description:Total RNAs were extracted from the hippocampus and prefrontal cortex of anxious mice. The transcriptome of the two brain regions were investigated using a custom made Agilent 8 x 15k features oligo microarray.

2010-12-31 | E-MEXP-2603 | biostudies-arrayexpress

Transcription profiling of human immature dendritic cells treated with Lipopolysaccharide (LPS) vs Taxol (paclitaxel)

Project description:Generation of monocyte-derived dendritic cells (DC). Immature DC were prepared from peripheral blood mononuclear cells (PBMCs) isolated from whole blood of healthy donors. A minimum of 3 individual donor blood was used for each experiment. PBMCs were incubated with anti-CD14 microbeads (Miltenyi Biotec, Bergish Gladbach, Germany) as specified in the manufacturer's instructions. The isolated monocytes were resuspended at 10^6 cells/ml in medium supplemented with 10% human AB serum, 100 ng/ml GM-CSF (Leucomax; Novartis, Camberley, UK) and 50 ng/ml IL-4 (Peprotech EC Ltd, London, UK), cultured in tissue culture flasks. On day 7 the cells were defined as immature DC with low/intermediate HLA-DR expression. Microarray analysis of the effects of Paclitaxel and LPS on DC. Total RNA was extracted from day 7 DC which had been treated for 2 h with 1 ug/ml LPS or 100 uM paclitaxel for 2 h then washed and returned to culture for 24 h. This was used as a template to generate Cy3-labelled cRNA, using the Low RNA Input Linear Amplification Kit (Agilent). This was used as a probe on the Whole Human Genome Microarray (4x44K) slide (Agilent). Slides were scanned using the Agilent scanner and data extracted using Feature Extraction Software 9.5.3 (Agilent). Subsequent data analysis was performed using GeneSpring GX software.

2009-11-27 | E-MEXP-2465 | biostudies-arrayexpress

Expression profiling, MDA-MB-231-luc2 response to TGFbeta2 and ST247

Project description:MDA-MB-231-luc2 cells were treated with TGFbeta2, ST247 or both

2017-06-01 | E-MTAB-1211 | biostudies-arrayexpress